Utilization of a PNA-peptide conjugate to induce a cancer protease-responsive RNAi effect

被引：2

作者：

Lee, Eun Kyung ^{[1
]}

Kim, Chan Woo ^{[2
]}

Kawanami, Hiroyuki ^{[1
]}

Kishimura, Akihiro ^{[1
,2
,3
,4
]}

Niidome, Takuro ^{[2
]}

Mori, Takeshi ^{[1
,2
,3
]}

Katayama, Yoshiki ^{[1
,2
,3
,4
,5
]}

机构：

[1] Kyushu Univ, Grad Sch Syst Life Sci, Nishi Ku, Fukuoka 8190395, Japan

[2] Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan

[3] Kyushu Univ, Ctr Future Chem, Nishi Ku, Fukuoka 8190395, Japan

[4] Kyushu Univ, Int Res Ctr Mol Syst, Nishi Ku, Fukuoka 8190395, Japan

[5] Kyushu Univ, Ctr Adv Med Innovat, Higashi Ku, Fukuoka 8128582, Japan

来源：

RSC ADVANCES | 2015年 / 5卷 / 104期

关键词：

APTAMER-SIRNA CHIMERAS; DOUBLE-STRANDED-RNA; RIBONUCLEOTIDE REDUCTASE; CATHEPSIN-B; M2; SUBUNIT; IN-VITRO; INTERFERENCE; DELIVERY; THERAPY; THERAPEUTICS;

D O I：

10.1039/c5ra17737e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Small interfering RNA (siRNA) is regarded as a promising tool for cancer therapy because of the wide applicability to various cancer-related genes. However, non-specific delivery of siRNA is one of the major causes of adverse effects. To access the issue, here we designed a new siRNA system which turns on RNAi responding to a cancer cell-specific protease, cathepsin B. The system uses a peptide nucleic acid (PNA)-peptide conjugate to provide this protease-responsive activation. The PNA-peptides were found to form hybrids with double-stranded RNAs with complementary protruding regions, which then affected the susceptibility of dsRNA to Dicer. The dsRNA/PNA-peptide hybrids were activated in cancer cells with a high cathepsin B activity to show RNAi.

引用

页码：85816 / 85821

页数：6

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