Comparative Secretome Analyses Using a Hollow Fiber Culture System with Label-Free Quantitative Proteomics Indicates the Influence of PARK7 on Cell Proliferation and Migration/Invasion in Lung Adenocarcinoma

被引：18

作者：

Chang, Ying-Hua ^{[1
,2
]}

Lee, Shu-Hui ^{[1
]}

Chang, Hua-Chien ^{[1
]}

Tseng, Yau-Lin ^{[3
]}

Lai, Wu-Wei ^{[3
]}

Liao, Chen-Chung ^{[4
]}

Tsay, Yeou-Guang ^{[5
]}

Liao, Pao-Chi ^{[1
,6
]}

机构：

[1] Natl Cheng Kung Univ, Coll Med, Dept Environm & Occupat Hlth, Tainan 70428, Taiwan

[2] Natl Cheng Kung Univ, Coll Med, Inst Clin Pharm & Pharmaceut Sci, Tainan 70428, Taiwan

[3] Natl Cheng Kung Univ Hosp, Dept Surg, Tainan 70428, Taiwan

[4] Natl Yang Ming Univ, Prote Res Ctr, Taipei 112, Taiwan

[5] Natl Yang Ming Univ, Inst Biochem & Mol Biol, Taipei 112, Taiwan

[6] Natl Cheng Kung Univ, Ctr Micro Nano Sci & Technol, Tainan 70428, Taiwan

来源：

JOURNAL OF PROTEOME RESEARCH | 2012年 / 11卷 / 11期

关键词：

hollow fiber culture system; label-free quantitative proteomics; PARK7; cancer metastasis; SIGNAL PEPTIDES; CANCER INVASION; IN-VIVO; DJ-1; PROTEIN; GROWTH; METASTASIS; EXPRESSION; SUPPRESSOR; PREDICTION;

D O I：

10.1021/pr300362g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

As the leading cause of cancer death worldwide, lung cancer lacks effective diagnosis tools and treatments to prevent its metastasis. Fortunately, secretome has clinical usages as biomarkers and protein drugs. To discover the secretome that influences lung adenocarcinoma metastasis, the hollow fiber culture (HFC) system was used along with label free proteomics approach to analyze cell secretomes between CL1-0 and CL1-5 cell lines, which exhibit low and high metastatic potentials. Among the 703 proteins quantified, 50 possessed different levels between CL1-0 and CL1-5. PARK7 was a primary focus because of the lack of research involving lung adenocarcinoma. The cell proliferation, migration, and invasion properties of CL1-0, CL1-5, and A549 cells were significantly diminished when the expression of their PARK7 proteins was reduced. Conversely, these functions were promoted when PARK7 was overexpressed in CL1-0. In clinical expression, PARK7 levels within tissue specimens and plasma samples were significantly higher in the cancer group. This represents the first time the HFC system has been used with label free quantification to discern the elements of metastasis in lung adenocarcinoma cell secretomes. Likewise, PARK7 has never been researched for its role in promoting. lung adenocarcinorna progression.

引用

页码：5167 / 5185

页数：19