Discovery of microbial natural products by activation of silent biosynthetic gene clusters

被引：707

作者：

Rutledge, Peter J. ^{[1
]}

Challis, Gregory L. ^{[2
]}

机构：

[1] Univ Sydney, Sch Chem, Sydney, NSW 2006, Australia

[2] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England

来源：

NATURE REVIEWS MICROBIOLOGY | 2015年 / 13卷 / 08期

基金：

英国生物技术与生命科学研究理事会; 英国医学研究理事会;

关键词：

STREPTOMYCES-COELICOLOR GENOME; SECONDARY METABOLISM; ANTIBIOTIC PRODUCTION; POLYKETIDE SYNTHASES; MASS-SPECTROMETRY; CRYPTIC POLYKETIDE; DRIVEN DISCOVERY; RARE-EARTH; PEPTIDE; BACTERIAL;

D O I：

10.1038/nrmicro3496

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Microorganisms produce a wealth of structurally diverse specialized metabolites with a remarkable range of biological activities and a wide variety of applications in medicine and agriculture, such as the treatment of infectious diseases and cancer, and the prevention of crop damage. Genomics has revealed that many microorganisms have far greater potential to produce specialized metabolites than was thought from classic bioactivity screens; however, realizing this potential has been hampered by the fact that many specialized metabolite biosynthetic gene clusters (BGCs) are not expressed in laboratory cultures. In this Review, we discuss the strategies that have been developed in bacteria and fungi to identify and induce the expression of such silent BGCs, and we briefly summarize methods for the isolation and structural characterization of their metabolic products.

引用

页码：509 / 523

页数：15

共 142 条

[1] SBSPKS: structure based sequence analysis of polyketide synthases [J].

Anand, Swadha ;

Prasad, M. V. R. ;

Yadav, Gitanjali ;

Kumar, Narendra ;

Shehara, Jyoti ;

Ansari, Md. Zeeshan ;

Mohanty, Debasisa .

NUCLEIC ACIDS RESEARCH, 2010, 38 :W487-W496

[2] Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature [J].

Arnison, Paul G. ;

Bibb, Mervyn J. ;

Bierbaum, Gabriele ;

Bowers, Albert A. ;

Bugni, Tim S. ;

Bulaj, Grzegorz ;

Camarero, Julio A. ;

Campopiano, Dominic J. ;

Challis, Gregory L. ;

Clardy, Jon ;

Cotter, Paul D. ;

Craik, David J. ;

Dawson, Michael ;

Dittmann, Elke ;

Donadio, Stefano ;

Dorrestein, Pieter C. ;

Entian, Karl-Dieter ;

Fischbach, Michael A. ;

Garavelli, John S. ;

Goeransson, Ulf ;

Gruber, Christian W. ;

Haft, Daniel H. ;

Hemscheidt, Thomas K. ;

Hertweck, Christian ;

Hill, Colin ;

Horswill, Alexander R. ;

Jaspars, Marcel ;

Kelly, Wendy L. ;

Klinman, Judith P. ;

Kuipers, Oscar P. ;

Link, A. James ;

Liu, Wen ;

Marahiel, Mohamed A. ;

Mitchell, Douglas A. ;

Moll, Gert N. ;

Moore, Bradley S. ;

Mueller, Rolf ;

Nair, Satish K. ;

Nes, Ingolf F. ;

Norris, Gillian E. ;

Olivera, Baldomero M. ;

Onaka, Hiroyasu ;

Patchett, Mark L. ;

Piel, Joern ;

Reaney, Martin J. T. ;

Rebuffat, Sylvie ;

Ross, R. Paul ;

Sahl, Hans-Georg ;

Schmidt, Eric W. ;

Selsted, Michael E. .

NATURAL PRODUCT REPORTS, 2013, 30 (01) :108-160

[3] Structural basis for transcription regulation by alarmone ppGpp [J].

Artsimovitch, I ;

Patlan, V ;

Sekine, SI ;

Vassylyeva, MN ;

Hosaka, T ;

Ochi, K ;

Yokoyama, S ;

Vassylyev, DG .

CELL, 2004, 117 (03) :299-310

[4] Structurally Diverse Chaetophenol Productions Induced by Chemically Mediated Epigenetic Manipulation of Fungal Gene Expression [J].

Asai, Teigo ;

Yamamoto, Takashi ;

Shirata, Naoki ;

Taniguchi, Tohru ;

Monde, Kenji ;

Fujii, Isao ;

Gomi, Katsuya ;

Oshima, Yoshiteru .

ORGANIC LETTERS, 2013, 15 (13) :3346-3349

[5] METHODS FOR IN SILICO PREDICTION OF MICROBIAL POLYKETIDE AND NONRIBOSOMAL PEPTIDE BIOSYNTHETIC PATHWAYS FROM DNA SEQUENCE DATA [J].

Bachmann, Brian O. ;

Ravel, Jacques .

COMPLEX ENZYMES IN MICROBIAL NATURAL PRODUCT BIOSYNTHESIS, PART A: OVERVIEW ARTICLES AND PEPTIDES, 2009, 458 :181-217

[6] VelB/VeA/LaeA complex coordinates light signal with fungal development and secondary metabolism [J].

Bayram, Oezguer ;

Krappmann, Sven ;

Ni, Min ;

Bok, Jin Woo ;

Helmstaedt, Kerstin ;

Valerius, Oliver ;

Braus-Stromeyer, Susanna ;

Kwon, Nak-Jung ;

Keller, Nancy P. ;

Yu, Jae-Hyuk ;

Braus, Gerhard H. .

SCIENCE, 2008, 320 (5882) :1504-1506

[7] Antiterminator-Mediated Unveiling of Cryptic Polythioamides in an Anaerobic Bacterium [J].

Behnken, Swantje ;

Lincke, Thorger ;

Kloss, Florian ;

Ishida, Keishi ;

Hertweck, Christian .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 51 (10) :2425-2428

[8] Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2) [J].

Bentley, SD ;

Chater, KF ;

Cerdeño-Tárraga, AM ;

Challis, GL ;

Thomson, NR ;

James, KD ;

Harris, DE ;

Quail, MA ;

Kieser, H ;

Harper, D ;

Bateman, A ;

Brown, S ;

Chandra, G ;

Chen, CW ;

Collins, M ;

Cronin, A ;

Fraser, A ;

Goble, A ;

Hidalgo, J ;

Hornsby, T ;

Howarth, S ;

Huang, CH ;

Kieser, T ;

Larke, L ;

Murphy, L ;

Oliver, K ;

O'Neil, S ;

Rabbinowitsch, E ;

Rajandream, MA ;

Rutherford, K ;

Rutter, S ;

Seeger, K ;

Saunders, D ;

Sharp, S ;

Squares, R ;

Squares, S ;

Taylor, K ;

Warren, T ;

Wietzorrek, A ;

Woodward, J ;

Barrell, BG ;

Parkhill, J ;

Hopwood, DA .

NATURE, 2002, 417 (6885) :141-147

[9] Genomics-driven discovery of PKS-NRPS hybrid metabolites from Aspergillus nidulans [J].

Bergmann, Sebastian ;

Schuemann, Julia ;

Scherlach, Kirstin ;

Lange, Corinna ;

Brakhage, Axel A. ;

Hertweck, Christian .

NATURE CHEMICAL BIOLOGY, 2007, 3 (04) :213-217

[10] Regulation of secondary metabolism in streptomycetes [J].

Bibb, MJ .

CURRENT OPINION IN MICROBIOLOGY, 2005, 8 (02) :208-215

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