SERIAL SECTIONING AND MULTISPECTRAL IMAGING SYSTEM FOR VERSATILE BIOMEDICAL APPLICATIONS

被引：0

作者：

Symvoulidis, P. ^{[1
,3
]}

Perez, Cruz C. ^{[1
]}

Schwaiger, M. ^{[2
]}

Ntziachristos, V ^{[1
,3
]}

Westmeyer, G. G. ^{[1
,2
]}

机构：

[1] Tech Univ Munich, Helmholtz Zentrum Munchen, Inst Biol & Med Imaging, D-80290 Munich, Germany

[2] Tech Univ Munich, Dept Nucl Med, D-80290 Munich, Germany

[3] Tech Univ Munich, Chair Biol Imaging, D-80290 Munich, Germany

来源：

2014 IEEE 11th International Symposium on Biomedical Imaging (ISBI) | 2014年

关键词：

cryo-microtome serial tissue sectioning; epifluorescence imaging; cryoslicer imaging; multispectral imaging; 3D visualization; IN-VIVO; TOMOGRAPHY;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Serial sectioning combined with microscopy provides high resolution volumetric data to complement in-vivo imaging modalities and aid ex-vivo diagnostics. We describe the design of a fully-automated cryomicrotome combined with a multispectral reflection and fluorescence imaging system that enables high-throughput analyses of biological specimens with a large field of view and cellular resolution while keeping the manufacturing and running costs low. We show the performance of the system for representative applications in high-resolution volumetric imaging of reporter animals and multispectral tissue analysis. Furthermore, we demonstrate the versatility of the economical imaging system in applications such as in vivo epifluorescence imaging, histology slide scanning, cell counting and gel electrophoresis documentation.

引用

页码：890 / 893

页数：4

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