Genomic Analysis of a New Serovar of Leptospira weilii Serogroup Manhao

被引：19
作者：
Xu, Yinghua [1 ]
Zheng, Huajun [2 ,3 ]
Zhang, Ying [1 ]
Wang, Yuezhu [3 ]
Zhang, Jinlong [1 ]
Li, Zhe [1 ]
Cui, Shenghui [1 ]
Xin, Xiaofang [1 ]
Ye, Qiang [1 ]
Chang, Yung-Fu [4 ]
Wang, Junzhi [1 ]
机构：
[1] Natl Inst Food & Drug Control, Key Lab Minist Hlth Res Qual Standardizat Biotech, Beijing, Peoples R China
[2] Fudan Univ, IRD, Shanghai Inst Planned Parenthood Res, Key Lab Reprod Regulat NPFPC, Shanghai, Peoples R China
[3] Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Zhangjiang Hitech Pk, Shanghai, Peoples R China
[4] Cornell Univ, Dept Populat Med & Diag Sci, Ithaca, NY 14850 USA
来源：
FRONTIERS IN MICROBIOLOGY | 2017年 / 8卷
关键词：
Leptospirosis; serogroup Manhao; new serovar; pathogenesis; genome; FIELD GEL-ELECTROPHORESIS; LPS BIOSYNTHETIC LOCI; INTERROGANS; VIRULENCE; SEQUENCE; IDENTIFICATION; EVOLUTION; DATABASE; GENES; NOV;
D O I：
10.3389/fmicb.2017.00149
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Leptospirosis, caused by pathogenic Leptospira spp., is recognized as an important emerging zoonotic disease throughout the world. In this study, multiple approaches were used to characterize the recently discovered serovar Heyan strain L231. This strain can infect guinea pigs and belonged to the pathogenic species L. wellii. Genome sequencing analysis revealed the draft genome of 4.2 M bp with a G+C content of 40.67% for strain L231, and a total of 4,794 ORFs were identified. The strain L231 genome was found to have a larger LPS biosynthesis locus than that of strains L. interrogans serovar Lai and L. borgpetersenii serovar Hardjobovis. Phylogenomic reconstructions showed that the evolutionary position of L. weilii serovar Heyan was different from that of other serovars from serogroup Manhao. These findings may lead us to a better understanding of Leptospira pathogenesis and evolution.
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页数：10
相关论文
共 61 条
[1]   GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions [J].
Besemer, J ;
Lomsadze, A ;
Borodovsky, M .
NUCLEIC ACIDS RESEARCH, 2001, 29 (12) :2607-2618
[2]   Leptospirosis: a zoonotic disease of global importance [J].
Bharti, AR ;
Nally, JE ;
Ricaldi, JN ;
Matthias, MA ;
Diaz, MM ;
Lovett, MA ;
Levett, PN ;
Gilman, RH ;
Willig, MR ;
Gotuzzo, E ;
Vinetz, JM .
LANCET INFECTIOUS DISEASES, 2003, 3 (12) :757-771
[3]   Leptospira mayottensis sp nov., a pathogenic species of the genus Leptospira isolated from humans [J].
Bourhy, Pascale ;
Collet, Louis ;
Brisse, Sylvain ;
Picardeau, Mathieu .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2014, 64 :4061-4067
[4]   Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies [J].
Brenner, DJ ;
Kaufmann, AF ;
Sulzer, KR ;
Steigerwalt, AG ;
Rogers, FC ;
Weyant, RS .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 :839-858
[5]   Genome reduction in Leptospira borgpetersenii reflects limited transmission potential [J].
Bulach, Dieter M. ;
Zuerner, Richard L. ;
Wilson, Peter ;
Seemann, Torsten ;
McGrath, Annette ;
Cullen, Paul A. ;
Davis, John ;
Johnson, Matthew ;
Kuczek, Elizabeth ;
Alt, David P. ;
Peterson-Burch, Brooke ;
Coppel, Ross L. ;
Rood, Julian I. ;
Davies, John K. ;
Adler, Ben .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (39) :14560-14565
[6]  
CROUT DHG, 1980, EUR J BIOCHEM, V106, P97
[7]   Mauve: Multiple alignment of conserved genomic sequence with rearrangements [J].
Darling, ACE ;
Mau, B ;
Blattner, FR ;
Perna, NT .
GENOME RESEARCH, 2004, 14 (07) :1394-1403
[8]  
de la Peña-Moctezuma A, 1999, FEMS MICROBIOL LETT, V177, P319, DOI 10.1111/j.1574-6968.1999.tb13749.x
[9]  
de la Peña-Moctezuma A, 2001, FEMS IMMUNOL MED MIC, V31, P73, DOI 10.1111/j.1574-695X.2001.tb01589.x
[10]  
Faine S., 1999, CURRENT TOPICS MICRO
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