Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section

被引：28

作者：

Tsai, TH ^{[1
]}

Lin, CY

Tsai, HJ

Chen, SY

Tai, SP

Lin, KH

Sun, CK

机构：

[1] Natl Taiwan Univ, Grad Inst Electroopt Engn, Taipei 10617, Taiwan

[2] Natl Taiwan Univ, Inst Mol & Cellular Biol, Taipei 10617, Taiwan

[3] Natl Taiwan Univ, Angiogenesis Res Ctr, Taipei 10617, Taiwan

[4] Natl Taiwan Univ, Dept Elect Engn, Taipei 10617, Taiwan

来源：

OPTICS LETTERS | 2006年 / 31卷 / 07期

关键词：

D O I：

10.1364/OL.31.000930

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wavelength in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated. (c) 2006 Optical Society of America.

引用

页码：930 / 932

页数：3

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