Characterization of a new obligately anaerobic thermophile, Thermoanaerobacter wiegelii sp nov

An obligately anaerobic, extremely thermophilic Thermoanaerobacter species was isolated from a freshwater pool formed from a geothermally heated (56 to 69 degrees C) water outlet in Government Gardens, Rotorua, New Zealand, This organism was a spore-forming, gram-negative, rod-shaped bacterium, Strain Rt8.B1(T) (= DSM 10319(T)) (T = type strain) fermented a wide variety of mono-, di-, and polysaccharides and produced ethanol, acetate, lactate, propionate, and hydrogen, Sugar alcohols were also fermented, but organic acids and amino acids were not utilized, On the basis of its morphological characteristics, DNA G+C content, obligately anaerobic, thermophilic, polysaccharolytic nature, and levels of 16S rRNA sequence homology, we propose that strain Rt8.B1(T) should be classified in the genus Thermoanaerobacter as a new species, Thermoanaerobacter wiegelii.