Biocompatibility Evaluation of EndoSequence Root Repair Paste in the Connective Tissue of Rats

Introduction: The aim of this study was to evaluate the subcutaneous connective tissue response to EndoSequence root repair paste (Brasseler, Savannah, GA) compared with mineral trioxide aggregate (MTA). Methods: Thirty-six Wistar rats each received 3 sterile tubes, containing 1 of the tested materials and control. The animals were killed 1, 3 and 6 weeks after implantation. The specimens were evaluated histologically for type of inflammation, intensity and extent of inflammatory cells, foreign body reaction, fibrous capsule thickness, perivascular fragments, calcific deposits and vascular congestion. Results: EndoSequence provoked severe inflammation after 1 week, which was significantly different from MTA and control (P < .05), with fragmented particles and foreign body reaction. MTA showed tissue-tolerance features almost comparable to control. Conclusions: EndoSequence was significantly more irritating than MTA and control at 1 and 3 weeks in terms of severity and extent of inflammation. After 6 weeks it displayed more biocompatible characteristics.