Diversity, phylogeny and host specificity of soybean and peanut bradyrhizobia

被引：25

作者：

Yang, Jiang Ke ^{[1
]}

Zhou, Jun Chu ^{[2
]}

机构：

[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China

[2] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

来源：

BIOLOGY AND FERTILITY OF SOILS | 2008年 / 44卷 / 06期

关键词：

soybean; peanut; bradyrhizobia; 16S rRNA RFLP; 16S-23S IGS RFLP;

D O I：

10.1007/s00374-008-0269-3

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

A study on the diversity, phylogeny, and host specificity of soybean (Glycine max L.) and peanut (Arachis hypogaea L.) bradyrhizobia was conducted based on the 16S ribosomal RNA (rRNA) restriction fragment length polymorphisms (RFLPs), 16S rRNA sequencing, and 16S-23S rRNA intergenetic spacer (IGS) RFLP assays. Based on 16S rRNA RFLP assay, tested bradyrhizobia were divided into five genotypes, which could be further clustered into five groups by IGS RFLP assays. According to the 16S rRNA sequencing, strains of IGS-II, IV, and V were phylogenetically related to Bradyrhizobium liaoningense, Bradyrhizobium japonicum, and Bradyrhizobium elkanii, while strains of IGS-Ic and IGS-III related to Bradyrhizobium yuanmingense and Bradyrhizobium canariense, respectively. All isolates could crossly nodulate Phaseolus vulgaris, forming small white nodules. Strains of IGS-II originally isolated from peanut could efficiently nodulate Glycine soja, and two strains isolated from soybean could also nodulate peanut.

引用

页码：843 / 851

页数：9

共 35 条

[21] European origin of Bradyrhizobium populations infecting lupins and serradella in soils of Western Australia and South Africa [J].

Stepkowski, T ;

Moulin, L ;

Krzyzanska, A ;

McInnes, A ;

Law, IJ ;

Howieson, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (11) :7041-7052

[22] Genetic diversity among Bradyrhizobium isolates that effectively nodulate peanut (Arachis hypogaea) [J].

Urtz, BE ;

Elkan, GH .

CANADIAN JOURNAL OF MICROBIOLOGY, 1996, 42 (11) :1121-1130

[23] Evolutionary relationships among the soybean bradyrhizobia reconstructed from 16S rRNA gene and internally transcribed spacer region sequence divergence [J].

van Berkum, P ;

Fuhrmann, JJ .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :2165-2172

[24] GENETIC AND PHENETIC ANALYSES OF BRADYRHIZOBIUM STRAINS NODULATING PEANUT (ARACHIS-HYPOGAEA L) ROOTS [J].

VANROSSUM, D ;

SCHUURMANS, FP ;

GILLIS, M ;

MUYOTCHA, A ;

VANVERSEVELD, HW ;

STOUTHAMER, AH ;

BOOGERD, FC .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (04) :1599-1609

[25]

Varanini Z., 2007, RHIZOSPHERE BIOCH OR, P297, DOI DOI 10.1201/9781420005585.CH11

[26]

VINCENT JM, 1970, MANUAL PRACTICAL STU, P73

[27] Bradyrhizobium canariense sp nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta [J].

Vinuesa, P ;

León-Barrios, M ;

Silva, C ;

Willems, A ;

Jarabo-Lorenzo, A ;

Pérez-Galdona, R ;

Werner, D ;

Martínez-Romero, E .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2005, 55 :569-575

[28]

Vinuesa P, 1998, APPL ENVIRON MICROB, V64, P2096

[29] AFLP fingerprint analysis of Bradyrhizobium strains isolated from Faidherbia albida and Aeschynomene species [J].

Willems, A ;

Doignon-Bourcier, F ;

Coopman, R ;

Hoste, B ;

de Lajudie, P ;

Gillis, M .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 2000, 23 (01) :137-147

[30]

Wilson K, 1989, CURRENT PROTOCOLS MO

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