Analysis of miR-376 RNA cluster members in the mouse inner ear

被引:15
作者
Yan, Denise [1 ]
Xing, Yazhi [2 ]
Ouyang, Xiaomei [1 ]
Zhu, Juhong [2 ]
Chen, Zheng-yi [3 ]
Lang, Hainan [2 ]
Liu, Xue Z. [1 ]
机构
[1] Univ Miami, Dept Otolaryngol, Miami, FL USA
[2] Med Univ S Carolina, Dept Pathol, Charleston, SC 29425 USA
[3] Harvard Univ, Sch Med, Dept Otolaryngol, Eaton Peabody Lab,Massachusetts Eye & Ear Infirm, Boston, MA 02115 USA
基金
美国国家卫生研究院;
关键词
A ? I editing; expression analysis; hearing loss; inner ear; microRNA-376; PRPS1; MICRORNA EXPRESSION; MUTATIONS; DICER; MORPHOGENESIS; BIOGENESIS; TARGETS; MIR-96; GENES; CELLS;
D O I
10.1111/j.1365-2613.2012.00840.x
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Mutations in phosphoribosyl pyrophosphate synthetase 1 (PRPS1) are associated with a spectrum of non-syndromic to syndromic hearing loss. PRPS1 transcript levels have been shown to be regulated by the microRNA-376 genes. The long primary RNA transcript of the miR-376 RNA cluster members undergo extensive and simultaneous A ? I editing at one or both of two specific sites (+4 and +44) in particular human and mouse tissues. The PRPS1 gene, which contains target sites for the edited version of miR-376a-5p within its 3'UTR, has been shown to be repressed in a tissue-specific manner. To investigate whether the transcription of Prps1 is regulated by miR-376 cluster members in the mouse inner ear, we first quantified the expression of the mature miR-376 RNAs by quantitative real-time-PCR. The spatio-temporal patterns of miR-376 expression were assessed by in situ hybridization. Finally, we examined whether A ?I editing of pri-miR-376 RNAs occurs in mouse inner ear by direct sequencing. Our data showed that the miR-376a-3p, b-3p, c-3p are present in mouse embryonic inner ears and intensive expression of miR-376a-3p/b-3p was detected in the sensory epithelia and ganglia of both auditory and vestibular portions of the inner ear. In adult inner ear, the expression of miR-376a-3p/b-3p is restricted within ganglion neurons of auditory and vestibular systems as well as the cells in the stria vascularis. Only unedited pri-miR-376 RNAs were detected in the cochlea suggesting that the activity of PRPS1 in the inner ear may not be regulated through the editing of miR-376 cluster.
引用
收藏
页码:450 / 457
页数:8
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