Engineering reprogrammable RNA-binding proteins for study and manipulation of the transcriptome

被引:15
作者
Abil, Zhanar [1 ]
Zhao, Huimin [1 ,2 ,3 ]
机构
[1] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Chem & Biomol Engn, Dept Bioengn, Dept Chem,Ctr Biophys & Computat Biol, Urbana, IL 61801 USA
[3] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA
基金
美国国家卫生研究院;
关键词
PENTATRICOPEPTIDE REPEAT PROTEINS; SINGLE-STRANDED RNA; STRUCTURAL BASIS; HUMAN PUMILIO; MODULAR RECOGNITION; MITOCHONDRIAL RNA; DNA-CLEAVAGE; PUF PROTEINS; SPECIFICITY; FAMILY;
D O I
10.1039/c5mb00289c
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
With the expanding interest in RNA biology, interest in artificial RNA-binding proteins (RBPs) is likewise increasing. RBPs can be designed in a modular fashion, whereby effector and RNA-binding domains are combined in chimeric proteins that exhibit both functions and can be applied for regulation of a broad range of biological processes. The elucidation of the RNA recognition code for Pumilio and fem-3 mRNA-binding factor (PUF) homology proteins allowed engineering of artificial RBPs for targeting endogenous mRNAs. In this review, we will focus on the recent advances in elucidating and reprogramming PUF domain specificity, update on several promising applications of PUF-based designer RBPs, and discuss some other domains that hold the potential to be used as the RNA-binding scaffolds for designer RBP engineering.
引用
收藏
页码:2658 / 2665
页数:8
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