A Toolbox for Tuberculosis (TB) Diagnosis: An Indian Multicentric Study (2006-2008). Evaluation of QuantiFERON-TB Gold in Tube for TB Diagnosis

被引:16
作者
Lagrange, Philippe H. [1 ]
Thangaraj, Satheesh K. [2 ,3 ]
Dayal, Rajeshwar [4 ]
Deshpande, Alaka [5 ]
Ganguly, Nirmal K. [6 ]
Girardi, Enrico [7 ]
Joshi, Beenu [8 ]
Katoch, Kiran [8 ]
Katoch, Vishwa M. [8 ]
Kumar, Manoj [9 ]
Lakshmi, Vemu [10 ]
Leportier, Marc [2 ,3 ]
Longuet, Christophe [11 ]
Malladi, Subbalaxmi V. S. [10 ]
Mukerjee, Deepali
Nair, Deepthi [12 ]
Raja, Alamelu [13 ]
Raman, Balambal [13 ]
Rodrigues, Camilla [14 ]
Sharma, Pratibha [12 ]
Singh, Amit [4 ]
Singh, Sarman [9 ]
Sodha, Archana [14 ]
Kabeer, Basirudeen Syed Ahamed [13 ]
Vernet, Guy [11 ]
Goletti, Delia
机构
[1] Univ Paris 07, Hop St Louis, AP HP, Microbiol Serv, Paris, France
[2] BioMerieux, Marcy Letoile, France
[3] BioMerieux, New Delhi, India
[4] SN Med Coll & Hosp, Agra, Uttar Pradesh, India
[5] Sir JJ Grp Govt Hosp & Grant Med Coll, Bombay, Maharashtra, India
[6] Indian Council Med Res, New Delhi, India
[7] L Spallanzani Natl Inst Infect Dis INMI, Dept Epidemiol & Preclin Res, Rome, Italy
[8] Natl JALMA Inst Leprosy & Other Mycrobacterial Di, Agra, Uttar Pradesh, India
[9] All India Inst Med Sci, New Delhi, India
[10] Nizams Inst Med Sci, Hyderabad, Andhra Pradesh, India
[11] Fdn Merieux, Lyon, France
[12] Safdarjang Hosp, New Delhi, India
[13] Natl Inst Res TB, Madras, Tamil Nadu, India
[14] PD Hinduja Hosp & Med Res Ctr, Microbiol Sect, Bombay, Maharashtra, India
来源
PLOS ONE | 2013年 / 8卷 / 09期
关键词
GAMMA RELEASE ASSAYS; ACTIVE TUBERCULOSIS; MYCOBACTERIUM-TUBERCULOSIS; INFECTION; TESTS; HIV; IDENTIFICATION; ACCURACY;
D O I
10.1371/journal.pone.0073579
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: The aim of this multicentric prospective study in India was to assess the performance of the QuantiFERON TB-Gold in tube (QFT-GIT), Tuberculin Skin Test (TST) and microbiological results as additional tools for diagnosing active tuberculosis (TB) and latent infection (LTBI) according to Human Immunodeficiency Virus (HIV) status. Methods: Individuals with and without active TB and HIV infection were enrolled between 2006-2008. QFT-GIT and TST results were analyzed per se and in combination with microbiological data. Results: Among the 276 individuals (96 active pulmonary TB and 180 no active TB) tested by QFT-GIT, 18 indeterminate results (6.5%) were found, more significantly numerous in the HIV-infected (15/92; 16.3%) than the HIV-uninfected (3/184; 1.6%)(p<0.0001). QFT-GIT sensitivity for active TB was 82.3% and 92.9% respectively after including or excluding indeterminate results. Clinical sensitivity was significantly lower in the HIV-infected (68.4%) than the HIV-uninfected (91.4%) patients (p = 0.0059). LTBI was detected in 49.3% of subjects without active TB but varied according to TB exposure. When the TST and QFT-GIT were concomitantly performed, the respective sensitivity for active TB diagnosis was 95.0% and 85.0% in the HIV-uninfected (p = 0.60), and 66.7% and 51.5% in the HIV-infected patients (p = 0.32). QFT-GIT and TST respective specificity for active TB in the HIV-uninfected was 25.0% and 57.1% (p = 0.028), and 64.8% and 83.3% in the HIV-infected (p = 0.047). In those with active TB, QFT-GIT results were not associated with microbiological parameters (smear grade, liquid culture status, time-to-positivity of culture) or clinical suspicion of active TB score (provided by the clinicians at enrollment). Combining microbiological tests with both immunological tests significantly increased sensitivity for active TB diagnosis (p = 0.0002), especially in the HIV-infected individuals (p = 0.0016). Conclusion: QFT-GIT and TST have similar diagnostic value for active TB diagnosis. In HIV-infected patients, combining microbiological tests with both immunological tests significantly increases the sensitivity for active TB diagnosis.
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页数:12
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