Centrosome duplication in mammalian somatic cells requires E2F and Cdk2-cyclin A

被引：415
作者：
Meraldi, P
Lukas, J
Fry, AM
Bartek, J
Nigg, EA
机构：
[1] Univ Geneva, Dept Mol Biol, CH-1211 Geneva 4, Switzerland
[2] Danish Canc Soc, Inst Canc Biol, Dept Cell Cycle & Canc, DK-2100 Copenhagen, Denmark
[3] Univ Leicester, Dept Biochem, Leicester LE1 7RH, Leics, England
来源：
NATURE CELL BIOLOGY | 1999年 / 1卷 / 02期
关键词：
D O I：
10.1038/10054
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Centrosome duplication is a key requirement for bipolar spindle formation and correct segregation of chromosomes during cell division. In a manner highly reminiscent of DNA replication, the centrosome must be duplicated once, and only once, in each cell cycle. How centrosome duplication is regulated and coordinated with other cell-cycle functions remains poorly understood. Here, we have established a centrosome duplication assay using mammalian somatic cells. We show that centrosome duplication requires the activation of E2F transcription factors and Cdk2-cyclin A activity.
引用
收藏
页码：88 / 93
页数：6
相关论文
共 49 条
[1]   DISSOCIATION OF CENTROSOME REPLICATION EVENTS FROM CYCLES OF DNA-SYNTHESIS AND MITOTIC DIVISION IN HYDROXYUREA-ARRESTED CHINESE-HAMSTER OVARY CELLS [J].
BALCZON, R ;
BAO, LM ;
ZIMMER, WE ;
BROWN, K ;
ZINKOWSKI, RP ;
BRINKLEY, BR .
JOURNAL OF CELL BIOLOGY, 1995, 130 (01) :105-115
[2]  
Boveri T., 1914, Zur Frage der Entstehung Maligner Tumoren
[3]   MICROTUBULE ORGANIZING CENTERS [J].
BRINKLEY, BR .
ANNUAL REVIEW OF CELL BIOLOGY, 1985, 1 :145-172
[4]   Reconstruction of the centrosome cycle from cryoelectron micrographs [J].
Chrétien, D ;
Buendia, B ;
Fuller, SD ;
Karsenti, E .
JOURNAL OF STRUCTURAL BIOLOGY, 1997, 120 (02) :117-133
[5]   ASSOCIATION OF HUMAN CYCLIN-E WITH A PERIODIC G(1)-S PHASE PROTEIN-KINASE [J].
DULIC, V ;
LEES, E ;
REED, SI .
SCIENCE, 1992, 257 (5078) :1958-1961
[6]   The regulation of E2F by pRB-family proteins [J].
Dyson, N .
GENES & DEVELOPMENT, 1998, 12 (15) :2245-2262
[7]   A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators [J].
Fry, AM ;
Meraldi, P ;
Nigg, EA .
EMBO JOURNAL, 1998, 17 (02) :470-481
[8]   Abnormal centrosome amplification in the absence of p53 [J].
Fukasawa, K ;
Choi, T ;
Kuriyama, R ;
Rulong, S ;
VandeVoude, GF .
SCIENCE, 1996, 271 (5256) :1744-1747
[9]   CENTROSOME DUPLICATION CONTINUES IN CYCLOHEXIMIDE-TREATED XENOPUS BLASTULAS IN THE ABSENCE OF A DETECTABLE CELL-CYCLE [J].
GARD, DL ;
HAFEZI, S ;
ZHANG, T ;
DOXSEY, SJ .
JOURNAL OF CELL BIOLOGY, 1990, 110 (06) :2033-2042
[10]   Cell cycle regulated expression of mammalian CDC6 is dependent on E2F [J].
Hateboer, G ;
Wobst, A ;
Petersen, BO ;
Le Cam, L ;
Vigo, E ;
Sardet, C ;
Helin, K .
MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (11) :6679-6697
12345