Characterization of Rv0394c gene encoding hyaluronidase and chondrosulfatase from Mycobacterium tuberculosis

被引：7

作者：

Yuan, Xianglian ^{[1
,2
]}

Chen, Liping ^{[1
]}

Deng, Xiaoxia ^{[2
]}

Cao, Jun ^{[1
]}

Yu, Shenye ^{[1
]}

Wang Quankai ^{[3
]}

Pang, Hai ^{[2
]}

Liu, Siguo ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Div Bacterial Dis, State Key Lab Vet Biotechnol, Harbin Vet Res Inst, Harbin 15000, Peoples R China

[2] Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China

[3] China Korea Res Inst Anim Sci, Changchun, Peoples R China

来源：

TUBERCULOSIS | 2013年 / 93卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Mycobacterium tuberculosis; Rv0394c; Hyaluronidase; Chondrosulfatase; STREPTOCOCCUS-PNEUMONIAE; BINDING PROTEINS; CELL; VIRULENCE; LYASE; LUNG; IDENTIFICATION; PURIFICATION; SEQUENCE; GROWTH;

D O I：

10.1016/j.tube.2013.01.004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

It has been demonstrated that Mycobacterium tuberculosis can utilize hyaluronan (HA) as an alternative carbon resource; however, the gene responsible for HA utilization has not been identified. We overexpressed the soluble product of the Rv0394c gene from M. tuberculosis H37Rv in Escherichia coli and purified it using affinity chromatography and anion exchange chromatography. The hyaluronidase and chondrosulfatase activities of the purified recombinant protein Rv0394c (rRv0394c) were detected and quantitatively measured. Analysis of nucleotide and derived amino acid sequences of the Rv0394c gene revealed that homologs of this gene were conserved in pathogenic mycobacteria, but absent in nonpathogenic mycobacteria. In the current study, we provide novel identification and characterization of a gene encoding hyaluronidase and chondrosulfatase in M. tuberculosis. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：296 / 300

页数：5

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