Bacterial type III secretion system as a protein delivery tool for a broad range of biomedical applications

被引：45

作者：

Bai, Fang ^{[1
]}

Li, Zhenpeng ^{[1
]}

Umezawa, Akihiro ^{[2
]}

Terada, Naohiro ^{[3
]}

Jin, Shouguang ^{[1
,4
]}

机构：

[1] Nankai Univ, Coll Life Sci, State Key Lab Med Chem Biol,Minist Educ, Key Lab Mol Microbiol & Technol,Dept Microbiol, Tianjin 300071, Peoples R China

[2] Natl Inst Child Hlth & Dev, Dept Reprod Biol, Tokyo 1578535, Japan

[3] Univ Florida, Coll Med, Dept Pathol, Gainesville, FL 32610 USA

[4] Univ Florida, Dept Mol Genet & Microbiol, Gainesville, FL 32610 USA

来源：

BIOTECHNOLOGY ADVANCES | 2018年 / 36卷 / 02期

基金：

美国国家卫生研究院; 对外科技合作项目（国际科技项目）; 美国国家科学基金会;

关键词：

Protein delivery; Type III secretion system; Vaccine; Cellular; Reprogramming; Genome editing; Stem cell differentiation; PLURIPOTENT STEM-CELLS; LIVE ATTENUATED SALMONELLA; VIVO ANTIGEN DELIVERY; PSEUDOMONAS-AERUGINOSA; YERSINIA-ENTEROCOLITICA; IN-VIVO; ANTITUMOR IMMUNOTHERAPY; PENETRATING PEPTIDES; SEROVAR TYPHIMURIUM; CYTOSINE DEAMINASE;

D O I：

10.1016/j.biotechadv.2018.01.016

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A protein delivery tool based on bacterial type III secretion system (T3SS) has been broadly applied in biomedical researches. In this review, we summarize various applications of the T3SS-mediate protein delivery which enables translocation of proteins directly into mammalian cells without protein purification. Some of the remarkable advancements include delivery of antigens for therapeutic vaccines, nucleases for genome editing, transcription factors for cellular reprogramming and stem cells differentiation, and signaling molecules for post translational proteomics studies. With continued improvement of the T3SS-mediated protein delivery tools, even wider application of the technology is anticipated.

引用

页码：482 / 493

页数：12

共 129 条

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