Chromosomal Organization and Sequence Diversity of Genes Encoding Lachrymatory Factor Synthase in Allium cepa L.

被引：11

作者：

Masamura, Noriya ^{[2
,3
]}

McCallum, John ^{[4
]}

Khrustaleva, Ludmila ^{[5
]}

Kenel, Fernand ^{[4
]}

Pither-Joyce, Meegham ^{[4
]}

Shono, Jinji ^{[3
]}

Suzuki, Go ^{[6
]}

Mukai, Yasuhiko ^{[6
]}

Yamauchi, Naoki ^{[1
,2
]}

Shigyo, Masayoshi ^{[1
,2
]}

机构：

[1] Yamaguchi Univ, Fac Agr, Yamaguchi 7538515, Japan

[2] Tottori Univ, United Grad Sch Agr Sci, Tottori 6808553, Japan

[3] House Foods Corp, Somatech Ctr, Chiba 2840033, Japan

[4] New Zealand Inst Plant & Food Res Ltd, Christchurch 8140, New Zealand

[5] Russian State Agr Univ, Moscow Timiryazev Agr Acad, Moscow 127550, Russia

[6] Osaka Kyoiku Univ, Div Nat Sci, Kashiwara 5828582, Japan

来源：

G3-GENES GENOMES GENETICS | 2012年 / 2卷 / 06期

基金：

日本科学技术振兴机构;

关键词：

Allium cepa; alien monosomic addition lines; genetic mapping; BAC-FISH; lachrymatory factor synthase; IN-SITU HYBRIDIZATION; ALIEN ADDITION LINES; EXPRESSED SEQUENCE; BAC FISH; ONION; REVEALS; DNA; GENOME; RICE; DUPLICATION;

D O I：

10.1534/g3.112.002592

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Lachrymatory factor synthase (LFS) catalyzes the formation of lachrymatory factor, one of the most distinctive traits of bulb onion (Allium cepa L.). Therefore, we used LFS as a model for a functional gene in a huge genome, and we examined the chromosomal organization of LFS in A. cepa by multiple approaches. The first-level analysis completed the chromosomal assignment of LFS gene to chromosome 5 of A. cepa via the use of a complete set of A. fistulosum-shallot (A. cepa L. Aggregatum group) monosomic addition lines. Subsequent use of an F-2 mapping population from the interspecific cross A. cepa x A. roylei confirmed the assignment of an LFS locus to this chromosome. Sequence comparison of two BAC clones bearing LFS genes, LFS amplicons from diverse germplasm, and expressed sequences from a doubled haploid line revealed variation consistent with duplicated LFS genes. Furthermore, the BAC-FISH study using the two BAC clones as a probe showed that LFS genes are localized in the proximal region of the long arm of the chromosome. These results suggested that LFS in A. cepa is transcribed from at least two loci and that they are localized on chromosome 5.

引用

页码：643 / 651

页数：9

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