Occurrence of classical swine fever virus in tissues of slaughtered pigs in different districts of Assam

被引：0

作者：

Sarma, D. K. ^{[1
]}

Sarma, S. ^{[1
]}

Tiwari, A. K. ^{[1
]}

Rajkhowa, S. ^{[1
]}

Meshram, D. J. ^{[1
]}

Singh, N. K. ^{[1
]}

Baruah, K. K. ^{[1
]}

机构：

[1] Assam Agr Univ, Coll Vet Sci, Dept Microbiol, Khanapara 781022, Guwahati, India

来源：

INDIAN JOURNAL OF VIROLOGY | 2007年 / 18卷 / 02期

关键词：

CSF virus; ELISA; BVDV; RT-PCR;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Tissue samples of pigs slaughtered for human consumption were examined for detection of classical swine fever (CSF) Virus antigen in Assam during April'2005 to March'2006. Out of 530 tissue samples consisting of kidney, spleen, tonsil, lymph node and intestine from 342 slaughtered pigs, 108 samples were found positive for CSF virus antigen in a sandwich ELISA. The highest number of kidney followed by spleen was found to be positive for the viral antigen. More number of samples collected in the months of July and October were found positive compared to other months. A few of the ELISA positive samples reacted with the monoclonal antibodies against the nonstructural protein (NS-3) of bovine viral diarrhea virus. Seven of the ELISA positive samples selected from different districts of the state was tested by RT-PCR and amplicons of 271bp could ne generated from 4 of the samples using pruners specific to the E2 region of the Virus. The present study confirms the presence of CSF virus in file tissues of slaughtered pigs in different districts of Assam.

引用

页码：61 / 64

页数：4

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