Development of a fluorescent-bead-based multiplex immunoassay to determine immunoglobulin G subclass responses to Neisseria meningitidis serogroup A and C polysaccharides

被引：44

作者：

de Voer, Richarda M. ^{[1
,2
]}

van der Klis, Fiona R. M. ^{[1
]}

Engels, Carla W. A. M. ^{[2
]}

Rijkers, Ger T. ^{[3
,4
]}

Sanders, Elisabeth A. ^{[3
]}

Berbers, Guy A. M. ^{[1
]}

机构：

[1] Natl Inst Publ Hlth, Lab Infect Dis & Screening, Bilthoven, Netherlands

[2] Univ Med Ctr, Dept Immunol, Utrecht, Netherlands

[3] Univ Med Ctr, Dept Pediat Immunol & Infect Dis, Utrecht, Netherlands

[4] St Antonius Hosp, Lab Med Microbiol & Immunol, Nieuwegein, Netherlands

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2008年 / 15卷 / 08期

关键词：

D O I：

10.1128/CVI.00478-07

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A fluorescent-particle-based multiplex flow cytometric immunoassay (MIA) for the detection of serum immunoglobulin G (IgG) and two IgG subclasses, IgG1 and IgG2, specific for Neisseria meningitidis serogroup A ( MenA) and C (MenC) polysaccharides ( PS) was developed. The assay comprised three separate duplex assays, one for the detection of the IgG response to MenA and MenC PS, another for the detection of the IgG1 response to MenA and MenC PS, and a third for the detection of the IgG2 response to MenA and MenC PS. Next, the three separate duplex assays were combined and analyzed as a hexaplex assay. No interference between monoplex, duplex, and hexaplex assays was observed, and the assay was found to have low intra- and interassay variation (< 9.0% and < 27%, respectively). Comparison of the meningococcal subclass MIA to the in-house enzyme-linked inmmunosorbent assays showed a good correlation (R >= 0.85) for each of the subclasses. We conclude that the hexaplex meningococcal subclass MIA is an easy and specific assay for the determination of anti-MenA and anti-MenC PS subclass IgG, requiring minimal amounts of serum to study IgG subclass responses to vaccines.

引用

页码：1188 / 1193

页数：6

共 27 条

[1] Method for simultaneous measurement of antibodies to 23 pneumococcal capsular polysaccharides [J].

Biagini, RE ;

Schlottmann, SA ;

Sammons, DL ;

Smith, JP ;

Snawder, JC ;

Striley, CAF ;

MacKenzie, BA ;

Weissman, DN .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2003, 10 (05) :744-750

[2] MULTICENTER COMPARISON OF LEVELS OF ANTIBODY TO THE NEISSERIA-MENINGITIDIS GROUP-A CAPSULAR POLYSACCHARIDE MEASURED BY USING AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY [J].

CARLONE, GM ;

FRASCH, CE ;

SIBER, GR ;

QUATAERT, S ;

GHEESLING, LL ;

TURNER, SH ;

PLIKAYTIS, BD ;

HELSEL, LO ;

DEWITT, WE ;

BIBB, WF ;

SWAMINATHAN, B ;

ARAKERE, G ;

THOMPSON, C ;

PHIPPS, D ;

MADORE, D ;

BROOME, CV .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (01) :154-159

[3] The kinetics and phenotype of the human B-cell response following immunization with a heptavalent pneumococcal-CRM197 conjugate vaccine [J].

Clutterbuck, Elizabeth A. ;

Salt, Penny ;

Oh, Sarah ;

Marchant, Arnaud ;

Beverley, Peter ;

Pollard, Andrew John .

IMMUNOLOGY, 2006, 119 (03) :328-337

[4] Optimization and validation of a multiplexed Luminex assay to quantify antibodies to neutralizing epitopes on human papillomaviruses 6, 11, 16, and 18 [J].

Dias, D ;

Van Doren, J ;

Schlottmann, S ;

Kelly, S ;

Puchalski, D ;

Ruiz, W ;

Boerckel, P ;

Kessler, J ;

Antonello, JM ;

Green, T ;

Brown, M ;

Smith, J ;

Chirmule, N ;

Barr, E ;

Jansen, KU ;

Esser, MT .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2005, 12 (08) :959-969

[5]

*EUR MED ASS, 1995, CGMPICH38195 EUR MED

[6] Immunoglobulin G subclass response to a meningococcal quadrivalent polysaccharide-diphtheria toxoid conjugate vaccine [J].

Findlow, H ;

Southern, J ;

Mabey, L ;

Balmer, P ;

Heyderman, RS ;

Auckland, C ;

Morris, R ;

Miller, E ;

Borrow, R .

CLINICAL AND VACCINE IMMUNOLOGY, 2006, 13 (04) :507-510

[7] MULTICENTER COMPARISON OF NEISSERIA-MENINGITIDIS SEROGROUP-C ANTI-CAPSULAR POLYSACCHARIDE ANTIBODY-LEVELS MEASURED BY A STANDARDIZED ENZYME-LINKED-IMMUNOSORBENT-ASSAY [J].

GHEESLING, LL ;

CARLONE, GM ;

PAIS, LB ;

HOLDER, PF ;

MASLANKA, SE ;

PLIKAYTIS, BD ;

ACHTMAN, M ;

DENSEN, P ;

FRASCH, CE ;

KAYHTY, K ;

MAYS, JP ;

NENCIONI, L ;

PEETERS, C ;

PHIPPS, DC ;

POOLMAN, JT ;

ROSENQVIST, E ;

SIBER, GR ;

THIESEN, B ;

TAI, J ;

THOMPSON, CM ;

VELLA, PP ;

WENGER, JD .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (06) :1475-1482

[8] Analogous IgG subclass response to pertussis toxin in vaccinated children, healthy or affected by whooping cough [J].

Giammanco, A ;

Taormina, S ;

Chiarini, A ;

Dardanoni, G ;

Stefanelli, P ;

Salmaso, S ;

Mastrantonio, P .

VACCINE, 2003, 21 (17-18) :1924-1931

[9] IGG SUBCLASS RESPONSE TO IMMUNIZATION WITH HEMOPHILUS-INFLUENZAE TYPE-B POLYSACCHARIDE OUTER MEMBRANE-PROTEIN CONJUGATE VACCINE [J].

GRANOFF, DM ;

WEINBERG, GA ;

SHACKELFORD, PG .

PEDIATRIC RESEARCH, 1988, 24 (02) :180-185

[10] ELISA METHODOLOGY FOR POLYSACCHARIDE ANTIGENS - PROTEIN COUPLING OF POLYSACCHARIDES FOR ADSORPTION TO PLASTIC TUBES [J].

GRAY, BM .

JOURNAL OF IMMUNOLOGICAL METHODS, 1979, 28 (1-2) :187-192

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