Complete sequence analysis of a gene (OS-9) ubiquitously expressed in human tissues and amplified in sarcomas

被引：0

作者：

Su, YA ^{[1
]}

Hutter, CM ^{[1
]}

Trent, JM ^{[1
]}

Meltzer, PS ^{[1
]}

机构：

[1] NATL CTR HUMAN GENOME RES,CANC GENET LAB,NIH,BETHESDA,MD 20892

来源：

MOLECULAR CARCINOGENESIS | 1996年 / 15卷 / 04期

关键词：

neoplasm; genetics; chromosome; 12q;

D O I：

10.1002/(SICI)1098-2744(199604)15:4<270::AID-MC4>3.3.CO;2-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amplification and overexpression of genes involved in cellular growth control occur frequently in human tumors. Using a chromosome microdissection-based hybrid-selection strategy, we recently identified two novel genes (OS-9 and OS-4) within 12q13-15, a region frequently amplified in human cancers. We now report further characterization of the full-length OS-9 cDNA sequence. This cDNA sequence consists of 2785 bp from which an open reading frame (ORF) with 667 amino-acid residues was deduced. The predicted polypeptide was water soluble and acidic. We also demonstrate that the OS-9 gene encoded a 2.8-kb mRNA transcribed in all 16 human tissues examined, suggesting that OS-9 is ubiquitously expressed in human tissues. OS-9 was coamplified with CDK4 in three of five sarcoma tissues. Homology analysis of the amino-acid sequence reveals significant similarities between OS-9 and two ORFs deduced from genomic sequences in Caenorhabditis elegans and Saccharomyces cerevisiae. The region of similarity extended over 200 residues (approximately one-third of each ORF), and eight cysteines were conserved in all three ORFs. These observations suggest that this region comprises a functional domain present in a novel evolutionarily conserved gene family defined by OS-9. (C) 1996 Wiley-Liss, Inc.*

引用

页码：270 / 275

页数：6

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