A conserved repetitive DNA element located in the centromeres of cereal chromosomes

被引：166
作者：
Jiang, JM
Nasuda, S
Dong, FG
Scherrer, CW
Woo, SS
Wing, RA
Gill, BS
Ward, DC
机构：
[1] KANSAS STATE UNIV,DEPT PLANT PATHOL,MANHATTAN,KS 66506
[2] UNIV WISCONSIN,CTR BIOTECHNOL,MADISON,WI 53706
[3] TEXAS A&M UNIV,DEPT PLANT & SOIL SCI,COLLEGE STN,TX 77843
[4] CLEMSON UNIV,DEPT AGRON & BIOL SCI,CLEMSON,SC 29634
[5] YALE UNIV,SCH MED,DEPT GENET,NEW HAVEN,CT 06510
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 24期
关键词：
D O I：
10.1073/pnas.93.24.14210
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Repetitive DNA sequences have been demonstrated to play an important role for centromere function of eukaryotic chromosomes, including those from fission yeast, Drosophila melanogaster, and humans, Here we report on the isolation of a repetitive DNA element located in the centromeric regions of cereal chromosomes, A 745-bp repetitive DNA clone, pSau3A9, was isolated from sorghum (Sorghum bicolor), This DNA element is located in the centromeric regions of all sorghum chromosomes, as demonstrated by fluorescence in situ hybridization. Repetitive DNA sequences homologous to pSau3A9 also are present in the centromeric regions of chromosomes from other cereal species, including rice, maize, wheat, barley, rye, and oats, Probe pSau3AB also hybridized to the centromeric region of B chromosomes from rye and maize, The repetitive nature and its conservation in distantly related plant species indicate that the pSau3A9 family may be associated with centromere function of cereal chromosomes. The absence of DNA sequences homologous to pSau3A9 in dicot species suggests a faster divergence of centromere-related sequences compared with the telomere-related sequences in plants.
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页码：14210 / 14213
页数：4
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