Evaluation of the Tetracore Orthopox BioThreat® antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens

被引：45

作者：

Townsend, Michael B. ^{[1
]}

MacNeil, Adam ^{[2
]}

Reynolds, Mary G. ^{[1
]}

Hughes, Christine M. ^{[1
]}

Olson, Victoria A. ^{[1
]}

Damon, Inger K. ^{[1
]}

Karem, Kevin L. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div High Consequence Pathogens & Pathol, Poxvirus & Rabies Branch, Atlanta, GA 30333 USA

[2] Ctr Dis Control & Prevent, Global Immunizat Div, CGH, Atlanta, GA 30333 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2013年 / 187卷 / 01期

关键词：

Monkeypox; Orthopoxvirus; BioThreat; Diagnostic; Rapid; HUMAN MONKEYPOX; VACCINIA-VIRUS; CONGO; PCR; INFECTION; IDENTIFICATION; OUTBREAK; STRAINS; COWPOX; RATIO;

D O I：

10.1016/j.jviromet.2012.08.023

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The commercially available Orthopox BioThreat (R) Alert assay for orthopoxvirus (OPV) detection is piloted. This antibody-based lateral-flow assay labels and captures OPV viral agents to detect their presence. Serial dilutions of cultured Vaccinia virus (VACV) and Monkeypox virus (MPXV) were used to evaluate the sensitivity of the Tetracore assay by visual and quantitative determinations; specificity was assessed using a small but diverse set of diagnostically relevant blinded samples from viral lesions submitted for routine OPV diagnostic testing. The BioThreat (R) Alert assay reproducibly detected samples at concentrations of 10(7) pfu/ml for VACV and MPXV and positively identified samples containing 10(6) pfu/ml in 4 of 7 independent experiments. The assay correctly identified 9 of 11 OPV clinical samples and had only one false positive when testing 11 non-OPV samples. Results suggest applicability for use of the BioThreat (R) Alert assay as a rapid screening assay and point of care diagnosis for suspect human monkeypox cases. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：37 / 42

页数：6

共 31 条

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