Phylogenetic analysis of Spiroplasmas from three freshwater crustaceans (Eriocheir sinensis, Procambarus clarkia and Penaeus vannamei) in China

被引：51

作者：

Bi, Keran ^{[1
]}

Huang, Hua ^{[1
]}

Gu, Wei ^{[1
]}

Wang, Junhai ^{[1
]}

Wang, Wen ^{[1
]}

机构：

[1] Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Biodivers & Biotechnol, Nanjing 210046, Peoples R China

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2008年 / 99卷 / 01期

关键词：

crustacean; 16S rRNA; 23S rRNA; 16S-23S rRNA intergenetic spacer regions (1SRs); gyrB; rpoB; genomic size; Western blot; Spiroplasma; phylogenetic analysis;

D O I：

10.1016/j.jip.2008.06.008

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Disease epizootics in freshwater culture crustaceans (crab, crayfish and shrimp) gained high attention recently in China, due to intensive developments of freshwater aquacultures. Spiroplasma was identified as a lethal pathogen of the above three freshwater Crustaceans in previous studies. Further characterization of these freshwater crustacean Spiroplasma strains were analyzed in the Current study. Phylogenetic position was investigated by analysis of partial nucleoticle sequences of 16S ribosomal RNA (rRNA), gyrB and rpoB genes, together with complete sequencing of 23S rRNA gene and 16S-23S rRNA intergenetic spacer regions (ISRs). Phylogenetic analysis of these sequences showed that the above-mentioned three freshwater crustacean Spiroplasma strains were identical and had a close relationship with Spiroplasma mirum. Furthermore, the genomic size, serological studies and experimental infection characteristics confirmed that three freshwater crustacean Spiroplasma strains are a single species other than traditional S. mirum. Therefore, these data Suggest that a single species of Spiroplasma infects all three investigated freshwater crustaceans in China, and is a potential candidate for a new species within the Spiroplasnia genus. These results provide critical information for the further investigations in fresh aquaculture epizootics related to tremor diseases, caused by this infectious agent. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：57 / 65

页数：9

共 42 条

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