Bioimaging of Hyaluronic Acid Derivatives Using Nanosized Carbon Dots

被引：146

作者：

Goh, Eun Ji ^{[1
]}

Kim, Ki Su ^{[1
]}

Kim, Yi Rang ^{[2
]}

Jung, Ho Sang ^{[1
]}

Beack, Songeun ^{[1
]}

Kong, Won Ho ^{[1
]}

Scarcelli, Giuliano ^{[3
,4
]}

Yun, Seok Hyun ^{[2
,3
,4
]}

Hahn, Sei Kwang ^{[1
,3
,4
]}

机构：

[1] Pohang Univ Sci & Technol POSTECH, Dept Mat Sci & Engn, Pohang 790784, Kyungbuk, South Korea

[2] Korea Adv Inst Sci & Technol, Grad Sch Nanosci & Technol WCU, Taejon 305701, South Korea

[3] Harvard Univ, Sch Med, Wellman Ctr Photomed, Cambridge, MA 02139 USA

[4] Massachusetts Gen Hosp, Cambridge, MA 02139 USA

来源：

BIOMACROMOLECULES | 2012年 / 13卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

QUANTUM DOTS; NANOPARTICLES; FLUORESCENCE; NANOTUBES; OXIDE; DELIVERY; BRIGHT;

D O I：

10.1021/bm300796q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescent nanosized carbon dots (Cdots) are an emerging bioimaging agent with excellent chemical inertness and marginal cytotoxicity in comparison to widely used semiconductor quantum dots. In this work, we report the application of Cdots for real time bioimaging of target specific delivery of hyaluronic acid (HA) derivatives. Polyethylene glycol (PEG) diamine-capped Cdots were synthesized by the pyrolysis of citric acid in a hot solvent The synthesized Cdots showed strong fluorescence under UV excitation with emission properties dependending on the excitation wavelength. HA-Cdot conjugates were synthesized by amide bond formation between amine groups of Cdot and carboxylic groups of HA. After confirmation of the negligible cytotoxicity of Cdots and HA Cdot conjugates, in vitro bioimaging was carried out for target specific intracellular delivery of the HA-Cdot conjugates by HA receptor mediated endocytosis. Furthermore, in vivo real-time bioimaging of Cdots and HA-Cdot conjugates exhibited the target specific delivery of HA-Cdot conjugates to the liver with abundant HA receptors. Taken together, we could confirm the feasibility of HA derivatives as a target specific drug delivery carrier for the treatment of liver diseases and Cdots as a promising bioimaging agent.

引用

页码：2554 / 2561

页数：8