Development of Specific Primers for the Molecular Detection of Bacterial Spot of Pepper and Tomato

被引：60

作者：

Koenraadt, H. ^{[1
]}

van Betteray, B. ^{[2
]}

Germain, R. ^{[3
]}

Hiddink, G. ^{[4
]}

Jones, J. B. ^{[5
]}

Oosterhof, J. ^{[6
]}

Rijlaarsdam, A. ^{[7
]}

Roorda, P. ^{[8
]}

Woudt, B. ^{[9
]}

机构：

[1] Naktuinbouw, Roelofarendsveen, Netherlands

[2] Nunhems Zaden BV, Haelen, Netherlands

[3] Clause Tezier, La Mentire, France

[4] ENZA Zaden BV, Enkhuizen, Netherlands

[5] Univ Florida, Gainesville, FL USA

[6] Rijk Zwaan, De Lier, Netherlands

[7] De Ruiter Seeds BV, Bergschenhoek, Netherlands

[8] Seminis Vegetable Seeds, Enkhuizen, Netherlands

[9] Syngenta Seeds, Enkhuizen, Netherlands

来源：

II INTERNATIONAL SYMPOSIUM ON TOMATO DISEASES | 2009年 / 808卷

关键词：

Xanthomonas euvesicatoria; Xanthomonas gardneri; Xanthomonas perforans; Xanthomonas vesicatoria; AFLP; PCR;

D O I：

10.17660/ActaHortic.2009.808.13

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Bacterial spot of tomato and pepper is an important seed-borne disease caused by a diverse group of bacteria currently classified as four species of X. euvesicatoria (Xe), Xanthomonas vesicatoria (Xv), X. perforans (Xp) and Xanthomonas gardneri (Xg). The objective of this research was to develop PCR primer sets that allow the specific detection and/or identification of Xe, Xv, Xp and Xg strains, in particular in seed assays. AFLP (R) was used to identify markers specific for each of the four species. Marker fragments were sequenced and potential primer sets were evaluated in PCR for their specificity. For all species specific primers were obtained.

引用

页码：99 / 102

页数：4

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