A static test was applied to study the biosorption of Mn(II) by immobilized dominant bacteria on the surface of chitosan cross-linked GAC(CCG) beads. The Citrobacter sp. was selected from the source water of the Shenyang first water treatment plant. Citrobacter sp. was immobilized on the surface of chitosan cross-linked GAC beads successfully. Langmuir models were applied to describe the isotherms. The Mn(II) removal rate was evaluated by the different dose rate and different contact time of the CCG beads. Results showed the dominant bacteria immobilized on chitosan cross-linked GAC beads were favourable adsorbers.