Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue

被引：112

作者：

Ingaramo, Maria ^{[1
]}

York, Andrew G. ^{[1
]}

Wawrzusin, Peter ^{[1
]}

Milberg, Oleg ^{[2
]}

Hong, Amy ^{[3
]}

Weigert, Roberto ^{[2
]}

Shroff, Hari ^{[1
]}

Patterson, George H. ^{[1
]}

机构：

[1] Natl Inst Biomed Imaging & Bioengn, NIH, Bethesda, MD 20892 USA

[2] Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD 20892 USA

[3] NHLBI, NIH, Bethesda, MD 20892 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 14期

基金：

美国国家卫生研究院;

关键词：

multiphoton; superresolution; DIFFRACTION-LIMIT; RESOLUTION LIMIT; FLUORESCENCE; PINHOLE; ARRAY; LIVE; BREAKING; SINGLE;

D O I：

10.1073/pnas.1314447111

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Multifocal structured illumination microscopy (MSIM) provides a twofold resolution enhancement beyond the diffraction limit at sample depths up to 50 mu m, but scattered and out-of-focus light in thick samples degrades MSIM performance. Here we implement MSIM with a microlens array to enable efficient two-photon excitation. Two-photon MSIM gives resolution-doubled images with better sectioning and contrast in thick scattering samples such as Caenorhabditis elegans embryos, Drosophila melanogaster larval salivary glands, and mouse liver tissue.

引用

页码：5254 / 5259

页数：6

共 31 条

[1] High-Resolution Intravital Microscopy
Andresen, Volker
Pollok, Karolin
Rinnenthal, Jan-Leo
Oehme, Laura
Guenther, Robert
Spiecker, Heinrich
Radbruch, Helena
Gerhard, Jenny
Sporbert, Anje
Cseresnyes, Zoltan
Hauser, Anja E.
Niesner, Raluca
[J]. PLOS ONE, 2012, 7 (12):
[2] Imaging intracellular fluorescent proteins at nanometer resolution
Betzig, Eric
Patterson, George H.
Sougrat, Rachid
Lindwasser, O. Wolf
Olenych, Scott
Bonifacino, Juan S.
Davidson, Michael W.
Lippincott-Schwartz, Jennifer
Hess, Harald F.
[J]. SCIENCE, 2006, 313 (5793) : 1642 - 1645
[3] Comparison of I5M and 4Pi-microscopy
Bewersdorf, J.
Schmidt, R.
Hell, S. W.
[J]. JOURNAL OF MICROSCOPY, 2006, 222 : 105 - 117
[4] Multifocal multiphoton microscopy
Bewersdorf, J
Pick, R
Hell, SW
[J]. OPTICS LETTERS, 1998, 23 (09) : 655 - 657
[5] Buist AH, 1998, J MICROSC-OXFORD, V192, P217, DOI 10.1046/j.1365-2818.1998.00431.x
[6] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY
DENK, W
STRICKLER, JH
WEBB, WW
[J]. SCIENCE, 1990, 248 (4951) : 73 - 76
[7] Edelstein Arthur, 2010, Curr Protoc Mol Biol, VChapter 14, DOI 10.1002/0471142727.mb1420s92
[8] Time multiplexing and parallelization in multifocal multiphoton microscopy
Egner, A
Hell, SW
[J]. JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2000, 17 (07): : 1192 - 1201
[9] Fast 100-nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast
Egner, A
Jakobs, S
Hell, SW
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (06) : 3370 - 3375
[10] Time-decorrelated multifocal array for multiphoton microscopy and micromachining
Fittinghoff, DN
Squier, JA
[J]. OPTICS LETTERS, 2000, 25 (16) : 1213 - 1215

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