Engineering lactococci and lactobacilli for human health

被引：130

作者：

Bermudez-Humaran, Luis G. ^{[1
,2
]}

Aubry, Camille ^{[1
,2
]}

Motta, Jean-Paul ^{[3
,4
,5
]}

Deraison, Celine ^{[3
,4
,5
]}

Steidler, Lothar ^{[6
]}

Vergnolle, Nathalie ^{[3
,4
,5
]}

Chatel, Jean-Marc ^{[1
,2
]}

Langella, Philippe ^{[1
,2
]}

机构：

[1] INRA, Commensal & Probiot Host Interact Lab, UMR Micalis 1319, F-78350 Jouy En Josas, France

[2] AgroParisTech, Micalis UMR1319, F-78350 Jouy En Josas, France

[3] Univ Toulouse 3, Univ Toulouse, CPTP, Toulouse, France

[4] Fac Med Toulouse, INSERM, U1043, F-31073 Toulouse, France

[5] CNRS, U5282, Toulouse, France

[6] ActoGeniX NV, B-9052 Zwijnaarde, Belgium

来源：

CURRENT OPINION IN MICROBIOLOGY | 2013年 / 16卷 / 03期

关键词：

LEUKOCYTE PROTEASE INHIBITOR; ANTIGEN-SPECIFIC TOLERANCE; LACTIC-ACID BACTERIA; SUPEROXIDE-DISMUTASE; EPITHELIAL-CELLS; MONOCYTOGENES INTERNALIN; LISTERIA-MONOCYTOGENES; DELIVERY VECTORS; MUCOSAL DELIVERY; MAMMALIAN-CELLS;

D O I：

10.1016/j.mib.2013.06.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Food-grade lactic acid bacteria (LAB) are good candidates for the development of oral vectors, and are attractive alternatives to attenuated pathogens, for mucosal delivery strategies. In this review, we summarize recent results on the use of LAB as mucosal delivery vectors for therapeutic proteins and DNA vaccines. Most of this work has been based on the model LAB, Lactococcus lactis, which is suitable for the heterologous expression of therapeutic proteins. Recombinant lactococci and lactobacilli strains expressing antiproteases and antioxidant enzymes have been tested successfully for their prophylactic and therapeutic effects in murine models of colitis. Recombinant lactococci secreting autoantigens have been found to be effective for the treatment of type 1 diabetes. Also, recombinant lactococci delivering DNA were able to prevent a bovine beta-lactoglobulin (BLG)-allergic reaction in mice. We believe that these various coherent findings demonstrate the potential value of using LAB, particularly lactococci and lactobacilli strains, to develop novel vectors for the therapeutic delivery of proteins to mucosa! surfaces. Further tests and in particular human clinical trials are now important next steps to conclude on the benefit of these approaches for human health.

引用

页码：278 / 283

页数：6

共 33 条

[1] An inducible surface presentation system improves cellular immunity against human papillomavirus type 16 E7 antigen in mice after nasal administration with recombinant lactococci [J].

Bermúdez-Humarán, LG ;

Cortes-Perez, NG ;

Le Loir, Y ;

Alcocer-González, JM ;

Tamez-Guerra, RS ;

de Oca-Luna, RM ;

Langella, P .

JOURNAL OF MEDICAL MICROBIOLOGY, 2004, 53 (05) :427-433

[2] Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines [J].

Bermudez-Humaran, Luis G. ;

Kharrat, Pascale ;

Chatel, Jean-Marc ;

Langella, Philippe .

MICROBIAL CELL FACTORIES, 2011, 10

[3] A phase I trial with Transgenic bacteria expressing interleukin-10 in Crohn's disease [J].

Braat, Henri ;

Rottiers, Pieter ;

Hommes, Daniel W. ;

Huyghebaert, Nathalie ;

Remaut, Erik ;

Remon, Jean-Paul ;

Van Deventer, Sander J. H. ;

Neirynck, Sabine ;

Peppelenbosch, Maikel P. ;

Steidler, Lothar .

CLINICAL GASTROENTEROLOGY AND HEPATOLOGY, 2006, 4 (06) :754-759

[4] Anti-inflammatory properties of Lactobacillus gasseri expressing manganese superoxide dismutase using the interleukin 10-deficient mouse model of colitis [J].

Carroll, Ian M. ;

Andrus, Jason M. ;

Bruno-Barcena, Jose M. ;

Klaenhammer, Todd R. ;

Hassan, Hosni M. ;

Threadgill, Deborah S. .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2007, 293 (04) :G729-G738

[5] Role for protease activity in visceral pain in irritable bowel syndrome [J].

Cenac, Nicolas ;

Andrews, Christopher N. ;

Holzhausen, Marinella ;

Chapman, Kevin ;

Cottrell, Graeme ;

Andrade-Gordon, Patricia ;

Steinhoff, Martin ;

Barbara, Giovanni ;

Beck, Paul ;

Bunnett, Nigel W. ;

Sharkey, Keith A. ;

Ferraz, Jose Geraldo P. ;

Shaffer, Eldon ;

Vergnolle, Nathalie .

JOURNAL OF CLINICAL INVESTIGATION, 2007, 117 (03) :636-647

[6] In vivo transfer of plasmid from food-grade transiting lactococci to murine epithelial cells [J].

Chatel, J-M ;

Pothelune, L. ;

Ah-Leung, S. ;

Corthier, G. ;

Wal, J-M ;

Langella, P. .

GENE THERAPY, 2008, 15 (16) :1184-1190

[7] In vitro and in vivo characterization of DNA delivery using recombinant Lactococcus lactis expressing a mutated form of L. monocytogenes Internalin A [J].

de Azevedo, Marcela ;

Karczewski, Jurgen ;

Lefevre, Francois ;

Azevedo, Vasco ;

Miyoshi, Anderson ;

Wells, Jerry M. ;

Langella, Philippe ;

Chatel, Jean-Marc .

BMC MICROBIOLOGY, 2012, 12

[8] Probiotics and the gut microbiota in intestinal health and disease [J].

Gareau, Melanie G. ;

Sherman, Philip M. ;

Walker, W. Allan .

NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY, 2010, 7 (09) :503-514

[9] Delay of the first onset of pouchitis by oral intake of the Probiotic strain Lactobacillus rhamnosus GG [J].

Gosselink, MP ;

Schouten, WR ;

van Lieshout, LMC ;

Hop, WCJ ;

Laman, JD ;

Ruseler-Van Embden, JGH .

DISEASES OF THE COLON & RECTUM, 2004, 47 (06) :876-884

[10] Functional gene transfer from intracellular bacteria to mammalian cells [J].

Grillot-Courvalin, C ;

Goussard, S ;

Huetz, F ;

Ojcius, DM ;

Courvalin, P .

NATURE BIOTECHNOLOGY, 1998, 16 (09) :862-866

← 1 2 3 4 →