The Hippo pathway regulates human megakaryocytic differentiation

被引：14

作者：

Lorthongpanich, Chanchao ^{[1
]}

Jiamvoraphong, Nittaya ^{[1
]}

Supraditaporn, Kantpitchar ^{[1
]}

Klaihmon, Phatchanat ^{[1
,2
]}

U-pratya, Yaowalak ^{[1
,3
]}

Issaragrisil, Surapol ^{[1
,3
]}

机构：

[1] Mahidol Univ, Siriraj Hosp, Siriraj Ctr Excellence Stem Cell Res, Fac Med, Bangkok, Thailand

[2] Mahidol Univ, Siriraj Hosp, Dept Immunol, Fac Med,Grad Program Immunol, Bangkok, Thailand

[3] Mahidol Univ, Siriraj Hosp, Fac Med, Div Hematol,Dept Med, Bangkok 10700, Thailand

来源：

THROMBOSIS AND HAEMOSTASIS | 2017年 / 117卷 / 01期

关键词：

Hippo pathway; LATS1/2; megakaryocyte; platelet; thrombopoiesis; LATS TUMOR-SUPPRESSOR; SOFT-TISSUE SARCOMAS; PROMOTER HYPERMETHYLATION; DROSOPHILA-MELANOGASTER; DOWN-REGULATION; HUMAN HOMOLOG; CELL-LINE; IN-VITRO; APOPTOSIS; CANCER;

D O I：

10.1160/TH16-07-0564

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The Hippo pathway is involved in several biological processes in both flies and mammals. Recent studies have shown that the Hippo pathway regulates Drosophila's haematopoiesis; however, understanding of its role in mammalian haematopoiesis is still limited. In flies, deletion of the Hippo component gene, Warts, affects crystal cell differentiation. We explored the role of the Hippo pathway in human haematopoiesis focusing on megakaryopoiesis. To investigate the role of LATS1/2 (a mammalian homolog of Warts) in human megakaryoblastic cell differentiation and platelet formation, megakaryoblastic cell (MEG-01) line was used as a model to gain insight into mechanism of the Hippo pathway in mammalian megakaryopoiesis. Effect of L41-51/2 on megakaryoblastic cell differentiation and platelet production were determined by functional changes. We found that depletion of LATS1/2 resulted in an increase of CD41(+) megakaryocytes with impaired platelet biogenesis. Our study shows that the Hippo signalling pathway plays a crucial role in human megakaryoblastic cell differentiation and thrombopoiesis.

引用

页码：116 / 126

页数：11

共 36 条

[21] Cytoskeletal regulation of platelet formation: Coordination of F-actin and microtubules [J].