Altered Innate Defenses in the Neonatal Gastrointestinal Tract in Response to Colonization by Neuropathogenic Escherichia coli

被引：41

作者：

Birchenough, George M. H. ^{[1
]}

Johansson, Malin E. V. ^{[2
]}

Stabler, Richard A. ^{[3
]}

Dalgakiran, Fatma ^{[1
]}

Hansson, Gunnar C. ^{[2
]}

Wren, Brendan W. ^{[3
]}

Luzio, J. Paul ^{[4
]}

Taylor, Peter W. ^{[1
]}

机构：

[1] UCL, Sch Pharm, London, England

[2] Univ Gothenburg, Mucin Biol Grp, Gothenburg, Sweden

[3] London Sch Hyg & Trop Med, London WC1, England

[4] Univ Cambridge, Cambridge Inst Med Res, Cambridge, England

来源：

INFECTION AND IMMUNITY | 2013年 / 81卷 / 09期

基金：

瑞典研究理事会; 英国医学研究理事会;

关键词：

K1 CAPSULAR POLYSACCHARIDE; PANETH CELLS; BACTERIAL-MENINGITIS; MOLECULAR ANALYSIS; POLYSIALIC ACID; EXPRESSION; MICROBIOTA; INFECTION; NEWBORN; HOST;

D O I：

10.1128/IAI.00268-13

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Two-day-old (P2), but not 9-day-old (P9), rat pups are susceptible to systemic infection following gastrointestinal colonization by Escherichia coli K1. Age dependency reflects the capacity of colonizing K1 to translocate from gastrointestinal (GI) tract to blood. A complex GI microbiota developed by P2, showed little variation over P2 to P9, and did not prevent stable K1 colonization. Substantial developmental expression was observed over P2 to P9, including upregulation of genes encoding components of the small intestinal (alpha-defensins Defa24 and Defa-rs1) and colonic (trefoil factor Tff2) mucus barrier. K1 colonization modulated expression of these peptides: developmental expression of Tff2 was dysregulated in P2 tissues and was accompanied by a decrease in mucin Muc2. Conversely, alpha-defensin genes were upregulated in P9 tissues. We propose that incomplete development of the mucus barrier during early neonatal life and the capacity of colonizing K1 to interfere with mucus barrier maturation provide opportunities for neuropathogen translocation into the bloodstream.

引用

页码：3264 / 3275

页数：12

共 75 条

[1] 6 WIDESPREAD BACTERIAL CLONES AMONG ESCHERICHIA-COLI K1 ISOLATES [J].