Crosslinking of enzymes for improved stability and performance

被引：131
作者：
Govardhan, CP [1 ]
机构：
[1] Altus Biol, Cambridge, MA 02139 USA
来源：
CURRENT OPINION IN BIOTECHNOLOGY | 1999年 / 10卷 / 04期
关键词：
D O I：
10.1016/S0958-1669(99)80060-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Stabilization of enzymes without diminishing and diluting their activity is crucial for their use as industrial catalysts. One practically proven approach involves crystallization of the enzyme (size 1-200 mu m) followed by subsequent crosslinking. In many cases, the resulting crosslinked enzyme crystals exhibit increased storage stability as well as stability in applications. The technology is complementary to protein engineering methods that aim at boosting the inherent stability of the enzyme.
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页码：331 / 335
页数：5
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