Glucosinolate Degradation Products, Isothiocyanates, Nitriles, and Thiocyanates, Induce Stomatal Closure Accompanied by Peroxidase-Mediated Reactive Oxygen Species Production in Arabidopsis thaliana

被引：66

作者：

Hossain, Mohammad Shakhawat ^{[1
]}

Ye, Wenxiu ^{[1
]}

Hossain, Mohammad Anowar ^{[1
]}

Okuma, Eiji ^{[1
]}

Uraji, Misugi ^{[1
]}

Nakamura, Yoshimasa ^{[1
]}

Mori, Izumi C. ^{[2
]}

Murata, Yoshiyuki ^{[1
]}

机构：

[1] Okayama Univ, Div Biosci, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan

[2] Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama 7100046, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2013年 / 77卷 / 05期

关键词：

allyl isothiocyanate; 3-butenenitrile; ethyl thiocyanate; glucosinolates; reactive oxygen species; GUARD-CELLS; HYPERSENSITIVE RESPONSE; SUPEROXIDE GENERATION; ALLYL ISOTHIOCYANATE; ABSCISIC-ACID; GENE FAMILY; INVOLVEMENT; ACTIVATION; MYROSINASE; INHIBITORS;

D O I：

10.1271/bbb.120928

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isothiocyanates, nitriles, and thiocyanates are degradation products of glucosinolates in crucifer plants. In this study, we investigated the stomatal response to ally! isothiocyanate (AITC), 3-butenenitrile (3BN), and ethyl thiocyanate (ESCN) in Arabidopsis. AITC, 3BN, and ESCN induced stomatal closure in the wild type and the atrbohD atrbohF mutant. Stomatal closure was inhibited by catalase and salicylhydroxamic acid (SHAM). The degradation products induced extracellular reactive oxygen species (ROS) production in the rosette leaves, and intracellular ROS accumulation, NO production, and cytosolic free calcium concentration ([Ca2+](cyt)) oscillations in guard cells, which were inhibited by SHAM. These results suggest that glucosinolate degradation products induce stomatal closure accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca2+](cyt) oscillation in Arabidopsis.

引用

页码：977 / 983

页数：7

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