Spotsizer: High-throughput quantitative analysis of microbial growth

被引：9

作者：

Bischof, Leanne ^{[1
]}

Prevorovsky, Martin ^{[2
,3
,4
]}

Rallis, Charalampos ^{[3
,4
]}

Jeffares, Daniel C. ^{[3
,4
]}

Arzhaeva, Yulia ^{[1
]}

Bahler, Jurg ^{[3
,4
]}

机构：

[1] CSIRO Digital Prod, N Ryde, NSW, Australia

[2] Charles Univ Prague, Dept Cell Biol, Fac Sci, Vinicna 5, Prague 12843 2, Czech Republic

[3] UCL, Dept Genet Evolut & Environm, London, England

[4] UCL, Inst Hlth Ageing, London, England

来源：

BIOTECHNIQUES | 2016年 / 61卷 / 04期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

colony array; yeast; bacteria; synthetic genetic array; microorganism; large-scale screen; YEAST SCHIZOSACCHAROMYCES-POMBE; FISSION YEAST; QUANTIFICATION; VISUALIZATION; DIVERSITY; ACCURATE; AGAR; CELL;

D O I：

10.2144/000114459

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microbial colony growth can serve as a useful readout in assays for studying complex genetic interactions or the effects of chemical compounds. Although computational tools for acquiring quantitative measurements of microbial colonies have been developed, their utility can be compromised by inflexible input image requirements, non-trivial installation procedures, or complicated operation. Here, we present the Spotsizer software tool for automated colony size measurements in images of robotically arrayed microbial colonies. Spotsizer features a convenient graphical user interface (GUI), has both single-image and batch-processing capabilities, and works with multiple input image formats and different colony grid types. We demonstrate how Spotsizer can be used for high-throughput quantitative analysis of fission yeast growth. The user-friendly Spotsizer tool provides rapid, accurate, and robust quantitative analyses of microbial growth in a high-throughput format. Spotsizer is freely available at https://data.csiro.au/dap/landingpage?pid=csiro:15330 under a proprietary CSIRO license.

引用

页码：191 / 201

页数：11

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