HOXA-10 gene expression in ectopic and eutopic endometrium tissues: Does it differ between fertile and infertile women with endometriosis?

被引:20
|
作者
Ozcan, Canan [1 ]
Ozdamar, Ozkan [2 ]
Gokbayrak, Merve Ertan [3 ]
Doger, Emek [1 ]
Cakiroglua, Yigit [1 ]
Cine, Naci [3 ]
机构
[1] Kocaeli Univ, Dept Obstet & Gynecol, Fac Med, Kocaeli, Turkey
[2] Istanbul Medeniyet Univ, Dept Obstet & Gynecol, Fac Med, Istanbul, Turkey
[3] Kocaeli Univ, Dept Genet, Fac Med, Kocaeli, Turkey
关键词
Endometriosis; Endometrioma; Infertility; HOXA-10; gene; IN-VITRO FERTILIZATION; IMPLANTATION FAILURE; ADENOMYOSIS; RECIPIENTS; PREGNANCY; OUTCOMES; HOXA10; IMPACT; WINDOW;
D O I
10.1016/j.ejogrb.2018.11.027
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To compare HOXA-10 gene expression in eutopic endometrium samples, between fertile and infertile endometriosis patients and the fertile control cases, and in endometrium and endometrioma specimens, between severe and moderate endometriosis cases. Study Design: Prospective clinical study included women without infertility and endometriosis (Group 1); women without infertility but with endometrioma (Group 2); and infertile women with endometrioma (Group 3). In addition, the Group 2 and 3 cohort were assessed based on the findings obtained during laparoscopy, based on the (rAFS) scoring, as women with a rAFS score of 16-40 were evaluated in Group A, whereas those with rAFS score above 40 were considered in Group B. HOXA-10 gene expression was evaluated in both secretory endometrium tissue and endometrioma specimens. Results: Eutopic endometrium samples from group 2 (reference gene = 0,680 vs. target gene = 0,362) and group 3 (reference gene = 0,641 vs. target gene = 0,183) patients revealed a 1,871-fold and 3,509-fold decrease in HOXA-10 gene expression, respectively, as compared to group 1. Endometrial HOXA-10 gene expression was 1,778-fold down-regulated in group 3 women (reference gene = 1,510 vs. target gene = 0,850), when compared to group 2. Both eutopic endometrium and endometrioma tissue samples from severe endometriosis patients revealed 1,259-fold (reference gene = 1,523 vs. target gene = 1,210) and 1,338-fold (reference gene = 1,274 vs. target gene = 0,952), down-regulation in HOXA-10 gene expressions, respectively, as compared to moderate cases. Conclusion: Endometrial HOXA-10 gene expression in women with endometriosis is significantly down-regulated than in those without endometriosis. Endometriosis patients with infertility have significantly lower levels of endometrial HOXA-10 gene expression than endometriosis without infertility; thus decreased expression of this gene may, directly or indirectly, be related with the endometriosis-associated infertility. Severe endometriosis cases express, in their both endometrium and endometrioma tissues, significantly lower levels of HOXA-10 gene than moderate endometriosis cases. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码:43 / 48
页数:6
相关论文
共 50 条
  • [41] COX-2 gene promoter DNA methylation status in eutopic and ectopic endometrium of Egyptian women with endometriosis
    Zidan, Haidy E.
    Rezk, Noha A.
    Alnemr, Amr Abd Almohsen
    Abd el Ghany, Amany M.
    JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2015, 112 : 63 - 67
  • [42] Fas and bcl-2 expression and association with apoptotic cells in eutopic and ectopic endometrium in women with endometriosis.
    Sbracia, M
    Scarpellini, F
    Zupi, E
    Marconi, D
    Mangoni, A
    DiTondo, U
    Romanini, C
    FERTILITY AND STERILITY, 1997, : P299 - P299
  • [43] Expression of HOXA10 Gene in Women with Endometriosis: A Systematic Review
    Lazim, Nurunnajah
    Elias, Marjanu Hikmah
    Sutaji, Zulazmi
    Abdul Karim, Abdul Kadir
    Abu, Mohammad Azrai
    Ugusman, Azizah
    Syafruddin, Saiful Effendi
    Mokhtar, Mohd Helmy
    Ahmad, Mohd Faizal
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (16)
  • [44] Increased Expression of TGF-β1 Contributes to the Downregulation of Progesterone Receptor Expression in the Eutopic Endometrium of Infertile Women with Minimal/Mild Endometriosis
    Wu, Lukanxuan
    Huang, Xin
    Wang, Ruiying
    Li, Yujing
    Zhu, Huili
    Ouyang, Yunwei
    Huang, Wei
    REPRODUCTIVE SCIENCES, 2023, 30 (12) : 3578 - 3589
  • [45] KISS1/KISS1R Expression in Eutopic and Ectopic Endometrium of Women Suffering from Endometriosis
    Makri, A.
    Msaouel, P.
    Petraki, C.
    Milingos, D.
    Protopapas, A.
    Liapi, A.
    Antsaklis, A.
    Magkou, C.
    Koutsilieris, M.
    IN VIVO, 2012, 26 (01): : 119 - 127
  • [46] Increased Expression of TGF-β1 Contributes to the Downregulation of Progesterone Receptor Expression in the Eutopic Endometrium of Infertile Women with Minimal/Mild Endometriosis
    Lukanxuan Wu
    Xin Huang
    Ruiying Wang
    Yujing Li
    Huili Zhu
    Yunwei Ouyang
    Wei Huang
    Reproductive Sciences, 2023, 30 : 3578 - 3589
  • [47] Differential Expression of CRH, UCN, CRHR1 and CRHR2 in Eutopic and Ectopic Endometrium of Women with Endometriosis
    Vergetaki, Aikaterini
    Jeschke, Udo
    Vrekoussis, Thomas
    Taliouri, Eirini
    Sabatini, Luca
    Papakonstanti, Evangelia A.
    Makrigiannakis, Antonis
    PLOS ONE, 2013, 8 (04):
  • [48] Aberrant expression of regulators of cell-fate found in eutopic endometrium is found in matched ectopic endometrium among women and in a baboon model of endometriosis
    Hapangama, D. K.
    Turner, M. A.
    Drury, J.
    Heathcote, L.
    Afshar, Y.
    Mavrogianis, P. A.
    Fazleabas, A. T.
    HUMAN REPRODUCTION, 2010, 25 (11) : 2840 - 2850
  • [49] Expression profile comparisons in eutopic endometrium between women with and without endometriosis during the window of implantation.
    Kao, LC
    Tulac, S
    Imani, B
    Yang, JP
    Lessey, BD
    Giudice, LC
    FERTILITY AND STERILITY, 2002, 77 (02) : S27 - S28
  • [50] Differential expression of microRNAs between eutopic and ectopic endometrium in ovarian endometriosis and possible role of microRNA 200c
    Gregnanin, I.
    Ferrara, M.
    Graziani, A.
    Surico, N.
    Filigheddu, N.
    FEBS JOURNAL, 2011, 278 : 86 - 86