HOXA-10 gene expression in ectopic and eutopic endometrium tissues: Does it differ between fertile and infertile women with endometriosis?

被引:20
|
作者
Ozcan, Canan [1 ]
Ozdamar, Ozkan [2 ]
Gokbayrak, Merve Ertan [3 ]
Doger, Emek [1 ]
Cakiroglua, Yigit [1 ]
Cine, Naci [3 ]
机构
[1] Kocaeli Univ, Dept Obstet & Gynecol, Fac Med, Kocaeli, Turkey
[2] Istanbul Medeniyet Univ, Dept Obstet & Gynecol, Fac Med, Istanbul, Turkey
[3] Kocaeli Univ, Dept Genet, Fac Med, Kocaeli, Turkey
关键词
Endometriosis; Endometrioma; Infertility; HOXA-10; gene; IN-VITRO FERTILIZATION; IMPLANTATION FAILURE; ADENOMYOSIS; RECIPIENTS; PREGNANCY; OUTCOMES; HOXA10; IMPACT; WINDOW;
D O I
10.1016/j.ejogrb.2018.11.027
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To compare HOXA-10 gene expression in eutopic endometrium samples, between fertile and infertile endometriosis patients and the fertile control cases, and in endometrium and endometrioma specimens, between severe and moderate endometriosis cases. Study Design: Prospective clinical study included women without infertility and endometriosis (Group 1); women without infertility but with endometrioma (Group 2); and infertile women with endometrioma (Group 3). In addition, the Group 2 and 3 cohort were assessed based on the findings obtained during laparoscopy, based on the (rAFS) scoring, as women with a rAFS score of 16-40 were evaluated in Group A, whereas those with rAFS score above 40 were considered in Group B. HOXA-10 gene expression was evaluated in both secretory endometrium tissue and endometrioma specimens. Results: Eutopic endometrium samples from group 2 (reference gene = 0,680 vs. target gene = 0,362) and group 3 (reference gene = 0,641 vs. target gene = 0,183) patients revealed a 1,871-fold and 3,509-fold decrease in HOXA-10 gene expression, respectively, as compared to group 1. Endometrial HOXA-10 gene expression was 1,778-fold down-regulated in group 3 women (reference gene = 1,510 vs. target gene = 0,850), when compared to group 2. Both eutopic endometrium and endometrioma tissue samples from severe endometriosis patients revealed 1,259-fold (reference gene = 1,523 vs. target gene = 1,210) and 1,338-fold (reference gene = 1,274 vs. target gene = 0,952), down-regulation in HOXA-10 gene expressions, respectively, as compared to moderate cases. Conclusion: Endometrial HOXA-10 gene expression in women with endometriosis is significantly down-regulated than in those without endometriosis. Endometriosis patients with infertility have significantly lower levels of endometrial HOXA-10 gene expression than endometriosis without infertility; thus decreased expression of this gene may, directly or indirectly, be related with the endometriosis-associated infertility. Severe endometriosis cases express, in their both endometrium and endometrioma tissues, significantly lower levels of HOXA-10 gene than moderate endometriosis cases. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码:43 / 48
页数:6
相关论文
共 50 条
  • [31] Harmonious pattern of HOXA10 gene expression with epigenetic aberration of its regulatory region in eutopic endometrium and ectopic endometriotic lesion of endometriosis patient during the menstrual cycle
    Samadieh, Y.
    Shahhosein, M.
    Mahdian, S.
    Ramezanali, F.
    Afsharian, P.
    Aflatoonian, R.
    FEBS JOURNAL, 2015, 282 : 62 - 62
  • [32] Aberrant expression of metastasis-inducing proteins in ectopic and matched eutopic endometrium of women with endometriosis: implications for the pathogenesis of endometriosis
    Hapangama, D. K.
    Raju, R. S.
    Valentijn, A. J.
    Barraclough, D.
    Hart, A.
    Turner, M. A.
    Platt-Higgins, A.
    Barraclough, R.
    Rudland, P. S.
    HUMAN REPRODUCTION, 2012, 27 (02) : 394 - 407
  • [33] HOXA-10 and E-cadherin expression in the endometrium of women with recurrent implantation failure and recurrent miscarriage
    Yang, Yihua
    Chen, Xiaoyan
    Saravelos, Sotirios H.
    Liu, Yingyu
    Huang, Jin
    Zhang, Jiamiao
    Li, Tin Chiu
    FERTILITY AND STERILITY, 2017, 107 (01) : 136 - +
  • [34] The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis
    Zhang, Longyu
    Liu, Ying
    Xu, Yuping
    Wu, Huan
    Wei, Zhaolian
    Cao, Yunxia
    INTERNATIONAL JOURNAL OF FERTILITY & STERILITY, 2015, 8 (04) : 429 - 436
  • [35] The Different Gene Expression Profile in the Eutopic and Ectopic Endometrium Sheds New Light on the Endometrial Seed in Endometriosis
    Riaz, Muhammad Assad
    Mecha, Ezekiel Onyonka
    Omwandho, Charles O. A.
    Zeppernick, Felix
    Meinhold-Heerlein, Ivo
    Konrad, Lutz
    BIOMEDICINES, 2024, 12 (06)
  • [36] Gene Expression Profile for Ectopic Versus Eutopic Endometrium Provides New Insights into Endometriosis Oncogenic Potential
    Borghese, Bruno
    Mondon, Francoise
    Noel, Jean-Christophe
    Fayt, Isabelle
    Mignot, Therese-Marie
    Vaiman, Daniel
    Chapron, Charles
    MOLECULAR ENDOCRINOLOGY, 2008, 22 (11) : 2557 - 2562
  • [37] Abnormal interleukin 1 receptor types I and II gene expression in eutopic and ectopic endometrial tissues of women with endometriosis
    Lawson, Christine
    Bourcier, Nathalie
    Al-Akourn, Mahera
    Maheux, Rodolphe
    Naud, Francoise
    Akoum, Ali
    JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2008, 77 (01) : 75 - 84
  • [38] Bioinformatics Analysis Reveals Novel Differentially Expressed Genes Between Ectopic and Eutopic Endometrium in Women with Endometriosis
    Sepideh Abdollahi
    Pantea Izadi
    Ghasem Azizi-Tabesh
    The Journal of Obstetrics and Gynecology of India, 2023, 73 : 115 - 123
  • [39] Bioinformatics Analysis Reveals Novel Differentially Expressed Genes Between Ectopic and Eutopic Endometrium in Women with Endometriosis
    Abdollahi, Sepideh
    Izadi, Pantea
    Azizi-Tabesh, Ghasem
    JOURNAL OF OBSTETRICS AND GYNECOLOGY OF INDIA, 2023, 73 (SUPPL 1): : 115 - 123
  • [40] Differential profile of transcripts detected by RNA-Seq in eutopic endometrium of infertile women with endometriosis and fertile controls during the implantation window
    Da Broi, M.
    Rocha, C. V., Jr.
    Placa, J.
    Peronni, K.
    Meola, J.
    Silva, W., Jr.
    Ferriani, R. A.
    Navarro, P. A.
    HUMAN REPRODUCTION, 2016, 31 : 282 - 283