The objective of this study was to evaluate the variety of Staphylococcus species isolated from milk samples of cow mastitis and to determine their resistance to antimicrobials. 516 strains of Staphylococcus were isolated from cow mastitis samples with total rate of 58.6%. In general, four species of staphylococci were identified. CPS were the most predominant: Staphylococcus aureus - 34.1 %, Staphylococcus hyicus - 26.34 % and Staphylococcus intermedius - 5.4 %. Staphylococcus epidermidis as CNS species was found as well in amount of 34.1 % from all staphylococci. S. aureus isolates demonstrated clinical resistance to streptomycin (78.3 %), clindamycin (22.3 %), neomycin (13.3 %), tetracycline (13.1 %) and erythromycin (12.8 %), whereas S. intermedius were resistant to streptomycin (78.6 %), tetracycline (3.6 %), enrofloxacin (14.3 %) and norfloxacin (21.4 %). 50 % of identified S. hyicus were resistant to streptomycin, 18.0 % to tetracycline, 17.2 % to enrofloxacin and 16.7 % to norfloxacin. 74.1 % of S. epidermidis isolates demonstrated resistance to streptomycin, 24.2 % to tetracycline, 18.6 % to lincomycin and 11.8 % to erythromycin. S. aureus, S. epidermidis were sensitive to fluoroquinolones. Different species staphylococcus were resistant to different antimicrobial substances. However, less resistances strains were CNS S. epidermidis (17.8 %), and more resistant CPS (20.9 %).