Polystyrene nanoparticles affecting blood coagulation

被引：80

作者：

Oslakovic, Cecilia ^{[1
]}

Cedervall, Tommy ^{[2
]}

Linse, Sara ^{[2
]}

Dahlback, Bjorn ^{[1
]}

机构：

[1] Lund Univ, Div Clin Chem, Dept Lab Med, Skane Univ Hosp, Malmo, Sweden

[2] Lund Univ, Ctr Chem, Lund, Sweden

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2012年 / 8卷 / 06期

基金：

瑞典研究理事会;

关键词：

Polystyrene; Nanoparticle; Blood coagulation; Nanosafety; Nanotoxicology; PLASMA-PROTEIN ADSORPTION; POLYMERIC NANOPARTICLES; ACTIVATION; PARTICLES; COMPLEX; CORONA;

D O I：

10.1016/j.nano.2011.12.001

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The association of nanoparticles (NPs) with blood coagulation proteins may influence the natural balance between pro-and anticoagulant pathways. We investigated whether polystyrene NPs, when added to human plasma, affected the generation of thrombin in plasma. Amine-modified NPs were found to decrease the thrombin formation due to binding of factors VII and IX to the NPs, which resulted in depletion of the respective protein in solution. In contrast, carboxyl-modified NPs were able to act as a surface for activation of the intrinsic pathway of blood coagulation in plasma. These results highlight the influence of NPs on a biologically important pathway. From the Clinical Editor: With the expected introduction of several nanoparticle-based therapeutic and diagnostic methods in the near future, their impact on basic homeostasis and obligatory biological processes needs to be carefully and accurately studied. In this study, the effects of polystyrene NP-s was investigated on blood coagulation pathways, and critically important biomodulatory effects were found. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：981 / 986

页数：6

共 25 条

[11] Functional groups on polystyrene model nanoparticles:: Influence on protein adsorption [J].

Gessner, A ;

Lieske, A ;

Paulke, BR ;

Müller, RH .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2003, 65A (03) :319-326

[12]

Gessner A, 2000, ELECTROPHORESIS, V21, P2438, DOI 10.1002/1522-2683(20000701)21:12<2438::AID-ELPS2438>3.3.CO

[13]

2-6

[14] Nanoparticles - Known and unknown health risks [J].

Hoet P.H.M. ;

Brüske-Hohlfeld I. ;

Salata O.V. .

Journal of Nanobiotechnology, 2 (1)

[15] Mouse recombinant protein C variants with enhanced membrane affinity and hyper-anticoagulant activity in mouse plasma [J].

Krisinger, Michael J. ;

Guo, Li Jun ;

Salvagno, Gian Luca ;

Guidi, Gian Cesare ;

Lippi, Giuseppe ;

Dahlback, Bjorn .

FEBS JOURNAL, 2009, 276 (22) :6586-6602

[16]

Lück M, 1998, J BIOMED MATER RES, V39, P478

[17] Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts [J].

Lundqvist, Martin ;

Stigler, Johannes ;

Elia, Giuliano ;

Lynch, Iseult ;

Cedervall, Tommy ;

Dawson, Kenneth A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (38) :14265-14270

[18]

Lynch I., 2006, Sci. STKE, V327, P14, DOI DOI 10.1126/STKE.3272006PE14

[19] The nanoparticle - protein complex as a biological entity; a complex fluids and surface science challenge for the 21st century [J].

Lynch, Iseult ;

Cedervall, Tommy ;

Lundqvist, Martin ;

Cabaleiro-Lago, Celia ;

Linse, Sara ;

Dawson, Kenneth A. .

ADVANCES IN COLLOID AND INTERFACE SCIENCE, 2007, 134-35 :167-174

[20] Platelet Polyphosphates Are Proinflammatory and Procoagulant Mediators In Vivo [J].

Muller, Felicitas ;

Mutch, Nicola J. ;

Schenk, Wolfdieter A. ;

Smith, Stephanie A. ;

Esterl, Lucie ;

Spronk, Henri M. ;

Schmidbauer, Stefan ;

Gahl, William A. ;

Morrissey, James H. ;

Renne, Thomas .

CELL, 2009, 139 (06) :1143-1156

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