Isolation and Characterization of 15 New Microsatellite Markers in Oncomelania hupensis, the Snail Intermediate Host of Schistosoma japonicum in Mainland China

被引:4
作者
Zhang, Li [1 ]
Li, Shizhu [1 ]
Wang, Qiang [1 ]
Qian, Yingjun [1 ]
Liu, Qin [1 ]
Yang, Pin [1 ]
Zhou, Xiaonong [1 ]
机构
[1] Natl Inst Parasit Dis, Chinese Ctr Dis Control & Prevent, Shanghai 200025, Peoples R China
基金
中国国家自然科学基金;
关键词
Oncomelania hupensis; microsatellites DNA; polymorphism; population genetics; NULL ALLELES; TRANSMISSION; GASTROPODA;
D O I
10.3390/ijms13055844
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum, which plays a key role during the transmission of schistosomiasis. It is mainly found in the Yangtze River valley and mountains or hills in southwest China. In this paper, we described 15 new microsatellite makers in O. hupensis. Polymorphism of each locus was assessed in 80 individuals from four wild populations (n = 20 per population). The number of alleles per locus ranged from 6 to 29, with an average of 15.8. The observed (H-O) and expected (H-E) heterozygosities varied from 0.397 to 0.851 and from 0.696 to 0.948, respectively. These microsatellite markers will be useful for population genetic studies and genome mapping in O. hupensis.
引用
收藏
页码:5844 / 5850
页数:7
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