A domain containing the Cdc42/Rac interactive binding (CRIB) region of p65(PAK) inhibits transcriptional activation and cell transformation mediated by the Ras-Rac pathway

被引：27
作者：
Osada, S [1 ]
Izawa, M [1 ]
Koyama, T [1 ]
Hirai, S [1 ]
Ohno, S [1 ]
机构：
[1] YOKOHAMA CITY UNIV,SCH MED,DEPT MOL BIOL,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN
来源：
FEBS LETTERS | 1997年 / 404卷 / 2-3期
关键词：
small GTP-binding protein; transcriptional activation; cell transformation; signal transduction;
D O I：
10.1016/S0014-5793(97)00139-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The molecular bases of the versatile functions of Rho-like GTPases are still unknown. Using luciferase assays with rat 3Y1 cells, we found that Rad is integrated downstream of Ras in the TRE (TPA response element) activation pathway. Coexpression of a mutant of p65(PAK), PAK/RD, lacking the kinase domain but containing the Cdc42/Rac interactive binding (CRIB) region, suppressed the TRE activation and cell transformation caused by constitutively activated forms of Ras (RasV12) and Rac1 (Rac1V12). PAK/RD is a good tool to investigate the signaling pathways in which Rac and Cdc42 are involved. (C) 1997 Federation of European Biochemical Societies.
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收藏
页码：227 / 233
页数：7
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