Distinct alternative splicing patterns of mediator subunit genes during endothelial progenitor cell differentiation

被引：13

作者：

Rienzo, Monica ^{[1
]}

Casamassimi, Amelia ^{[1
]}

Schiano, Concetta ^{[1
]}

Grimaldi, Vincenzo ^{[1
]}

Infante, Teresa ^{[2
]}

Napoli, Claudio ^{[1
,2
]}

机构：

[1] Univ Naples 2, UOC Immunohematol Transfus Med & Transplant Immun, Excellence Res Ctr Cardiovasc Dis,Reg Reference L, Dept Gen Pathol,Chair Clin Pathol,AOU,Sch Med 1, I-80138 Naples, Italy

[2] IRCCS SDN Fdn, I-80134 Naples, Italy

来源：

BIOCHIMIE | 2012年 / 94卷 / 08期

关键词：

Mediator complex; Alternative splicing; MED12; MED19; Endothelial progenitor cells; CD133 positive cells;

D O I：

10.1016/j.biochi.2012.04.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mediator (MED) is a fundamental component of the RNA polymerase II-mediated transcription machinery playing a pivotal role in the regulation of eukaryotic mRNA synthesis. Human MED complexes contain at least 30 distinct MED subunits. Our previous study, aimed to analyse MED complex during the pattern of endothelial progenitor cells (EPCs) differentiation, found an alternative transcript of MED30 subunit expressed only in circulating immature progenitor cells. Here, we report two novel transcripts of MED12 and MED19 subunits both generated by alternative splicing and displaying similar expression patterns, thereby indicating their involvement during endothelial cell differentiation. (C) 2012 Elsevier Masson SAS. All rights reserved.

引用

页码：1828 / 1832

页数：5

共 10 条

[1] Mediator complexes and eukaryotic transcription regulation: An overview
Casamassimi, Amelia
Napoli, Claudio
[J]. BIOCHIMIE, 2007, 89 (12) : 1439 - 1446
[2] Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches
Chen, Mo
Manley, James L.
[J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2009, 10 (11) : 741 - 754
[3] miR-148 targets human DNMT3b protein coding region
Duursma, Anja M.
Kedde, Martijn
Schrier, Mariette
Le Sage, Carlos
Agami, Reuven
[J]. RNA, 2008, 14 (05) : 872 - 877
[4] Functional consequences of developmentally regulated alternative splicing
Kalsotra, Auinash
Cooper, Thomas A.
[J]. NATURE REVIEWS GENETICS, 2011, 12 (10) : 715 - 729
[5] Unraveling framework of the ancestral Mediator complex in human diseases
Napoli, Claudio
Sessa, Marcella
Infante, Teresa
Casamassimi, Amelia
[J]. BIOCHIMIE, 2012, 94 (03) : 579 - 587
[6] Mediator subunits: Gene expression pattern, a novel transcript identification and nuclear localization in human endothelial progenitor cells
Rienzo, Monica
Nagel, Jens
Casamassimi, Amelia
Giovane, Alfonso
Dietzel, Steffen
Napoli, Claudio
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 2010, 1799 (07): : 487 - 495
[7] A ceRNA Hypothesis: The Rosetta Stone of a Hidden RNA Language?
Salmena, Leonardo
Poliseno, Laura
Tay, Yvonne
Kats, Lev
Pandolfi, Pier Paolo
[J]. CELL, 2011, 146 (03) : 353 - 358
[8] Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation
Salomonis, Nathan
Schlieve, Christopher R.
Pereira, Laura
Wahlquist, Christine
Colas, Alexandre
Zambon, Alexander C.
Vranizan, Karen
Spindler, Matthew J.
Pico, Alexander R.
Cline, Melissa S.
Clark, Tyson A.
Williams, Alan
Blume, John E.
Samal, Eva
Mercola, Mark
Merrill, Bradley J.
Conklin, Bruce R.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (23) : 10514 - 10519
[9] Role for Med12 in Regulation of Nanog and Nanog Target Genes
Tutter, Antonin V.
Kowalski, Michael P.
Baltus, Gretchen A.
Iourgenko, Vadim
Labow, Mark
Li, En
Kadam, Shilpa
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (06) : 3709 - 3718
[10] The pathobiology of splicing
Ward, Amanda J.
Cooper, Thomas A.
[J]. JOURNAL OF PATHOLOGY, 2010, 220 (02) : 152 - 163

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