G Protein-Coupled Receptor Transactivation: From Molecules to Mice

被引：13
作者：
Jonas, Kim C. [1 ]
Rivero-Mueller, Adolfo [2 ]
Huhtaniemi, Ilpo T. [1 ,2 ]
Hanyaloglu, Aylin C. [1 ]
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Inst Reprod & Dev Biol, Dept Surg & Canc, London, England
[2] Univ Turku, Dept Physiol, Inst Biomed, Turku, Finland
来源：
RECEPTOR-RECEPTOR INTERACTIONS | 2013年 / 117卷
基金：
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词：
HORMONE-RECEPTORS; GONADOTROPIN RECEPTORS; LH RECEPTOR; ACTIVATION; RESCUE; HOMODIMERIZATION; MAINTENANCE; FRAGMENTS; MUTANTS;
D O I：
10.1016/B978-0-12-408143-7.00023-2
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
G protein-coupled receptors (GPCRs) mediate a diverse range of physiological functions via activation of complex signaling systems. Organization of GPCRs in to dimers and oligomers provides a mechanism for both signal diversity and specificity in cellular responses, yet our understanding of the physiological significance of dimerization, particularly homodimerization, has not been forthcoming. This chapter will describe how we have investigated the physiological importance of GPCR homodimerization, using the luteinizing hormone/chorionic gonadotropin receptor as a model GPCR. Using transactivation as a mode of assessing receptor dimerization, we describe our cellular system and functional assays for assessment of transactivation in vitro and detail our strategy for generating a mouse model to assess GPCR transactivation in vivo.
引用
收藏
页码：433 / 450
页数：18
相关论文
共 50 条
  • [41] Deficiency of Both Farnesoid X Receptor and Takeda G Protein-Coupled Receptor 5 Exacerbated Liver Fibrosis in Mice
    Ferrell, Jessica M.
    Pathak, Preeti
    Boehme, Shannon
    Gilliland, Tricia
    Chiang, John Y. L.
    HEPATOLOGY, 2019, 70 (03) : 955 - 970
  • [42] Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor
    Gulati, Sahil
    Jin, Hui
    Masuho, Ikuo
    Orban, Tivadar
    Cai, Yuan
    Pardon, Els
    Martemyanov, Kirill A.
    Kiser, Philip D.
    Stewart, Phoebe L.
    Ford, Christopher P.
    Steyaert, Jan
    Palczewski, Krzysztof
    NATURE COMMUNICATIONS, 2018, 9
  • [43] Ligand binding to a G protein-coupled receptor captured in a mass spectrometer
    Yen, Hsin-Yung
    Hopper, Jonathan T. S.
    Liko, Idlir
    Allison, Timothy M.
    Zhu, Ya
    Wang, Dejian
    Stegmann, Monika
    Mohammed, Shabaz
    Wu, Beili
    Robinson, Carol V.
    SCIENCE ADVANCES, 2017, 3 (06):
  • [44] The role of water molecules in phototransduction of retinal proteins and G protein-coupled receptors
    Lesca, Elena
    Panneels, Valerie
    Schertler, Gebhard F. X.
    FARADAY DISCUSSIONS, 2018, 207 : 27 - 37
  • [45] Molecular mechanism of biased signaling in a prototypical G protein-coupled receptor
    Suomivuori, Carl-Mikael
    Latorraca, Naomi R.
    Wingler, Laura M.
    Eismann, Stephan
    King, Matthew C.
    Kleinhenz, Alissa L. W.
    Skiba, Meredith A.
    Staus, Dean P.
    Kruse, Andrew C.
    Lefkowitz, Robert J.
    Dror, Ron O.
    SCIENCE, 2020, 367 (6480) : 881 - +
  • [46] Overexpression of Diacylglycerol Kinase η Enhances Gαq-Coupled G Protein-Coupled Receptor Signaling
    Rittiner, Joseph E.
    Brings, Victoria E.
    Zylka, Mark J.
    MOLECULAR PHARMACOLOGY, 2014, 85 (05) : 800 - 810
  • [47] The role of G protein-coupled estrogen receptor 1 on neurological disorders
    Roque, C.
    Mendes-Oliveira, J.
    Duarte-Chendo, C.
    Baltazar, G.
    FRONTIERS IN NEUROENDOCRINOLOGY, 2019, 55
  • [48] G Protein-Coupled Receptor Signaling to Kir Channels in Xenopus Oocytes
    Hatcher-Solis, Candice
    Fribourg, Miguel
    Spyridaki, Katerina
    Younkin, Jason
    Ellaithy, Amr
    Xiang, Guoqing
    Liapakis, George
    Gonzalez-Maeso, Javier
    Zhang, Hailin
    Cui, Meng
    Logothetis, Diomedes E.
    CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2014, 15 (10) : 987 - 995
  • [49] Genetically Targeted Optical Control of an Endogenous G Protein-Coupled Receptor
    Donthamsetti, Prashant C.
    Broichhagen, Johannes
    Vyklicky, Vojtech
    Stanley, Cherise
    Fu, Zhu
    Visel, Meike
    Levitz, Joshua L.
    Javitch, Jonathan A.
    Trauner, Dirk
    Isacoff, Ehud Y.
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2019, 141 (29) : 11522 - 11530
  • [50] G protein-coupled receptor modulation with pepducins: moving closer to the clinic
    Dimond, Patricia
    Carlson, Kenneth
    Bouvier, Michel
    Gerard, Craig
    Xu, Lei
    Covic, Lidija
    Agarwal, Anika
    Ernst, Oliver P.
    Janz, Jay M.
    Schwartz, Thue W.
    Gardella, Thomas J.
    Milligan, Graeme
    Kuliopulos, Athan
    Sakmar, Thomas P.
    Hunt, Stephen W., III
    ANNALS MEETING REPORTS, 2011, 1226 : 34 - 49
12345