G Protein-Coupled Receptor Transactivation: From Molecules to Mice

被引：13
作者：
Jonas, Kim C. [1 ]
Rivero-Mueller, Adolfo [2 ]
Huhtaniemi, Ilpo T. [1 ,2 ]
Hanyaloglu, Aylin C. [1 ]
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Inst Reprod & Dev Biol, Dept Surg & Canc, London, England
[2] Univ Turku, Dept Physiol, Inst Biomed, Turku, Finland
来源：
RECEPTOR-RECEPTOR INTERACTIONS | 2013年 / 117卷
基金：
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词：
HORMONE-RECEPTORS; GONADOTROPIN RECEPTORS; LH RECEPTOR; ACTIVATION; RESCUE; HOMODIMERIZATION; MAINTENANCE; FRAGMENTS; MUTANTS;
D O I：
10.1016/B978-0-12-408143-7.00023-2
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
G protein-coupled receptors (GPCRs) mediate a diverse range of physiological functions via activation of complex signaling systems. Organization of GPCRs in to dimers and oligomers provides a mechanism for both signal diversity and specificity in cellular responses, yet our understanding of the physiological significance of dimerization, particularly homodimerization, has not been forthcoming. This chapter will describe how we have investigated the physiological importance of GPCR homodimerization, using the luteinizing hormone/chorionic gonadotropin receptor as a model GPCR. Using transactivation as a mode of assessing receptor dimerization, we describe our cellular system and functional assays for assessment of transactivation in vitro and detail our strategy for generating a mouse model to assess GPCR transactivation in vivo.
引用
收藏
页码：433 / 450
页数：18
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