Quantitative analysis of the flexibility effect of cisplatin on circular DNA

被引：5
作者：
Ji, Chao [1 ]
Zhang, Lingyun [1 ]
Wang, Peng-Ye [1 ]
机构：
[1] Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Key Lab Soft Matter Phys, Beijing 100190, Peoples R China
来源：
PHYSICAL REVIEW E | 2013年 / 88卷 / 04期
基金：
中国国家自然科学基金;
关键词：
SCANNING FORCE MICROSCOPY; COMPLEXES; BINDING; RECOGNITION; POLYMERASE; MOLECULES; ADDUCTS; LIGANDS; PBR322; PD(II);
D O I：
10.1103/PhysRevE.88.042703
中图分类号：
O35 [流体力学]; O53 [等离子体物理学];
学科分类号：
070204 ; 080103 ; 080704 ;
摘要：
We study the effects of cisplatin on the circular configuration of DNA using atomic force microscopy (AFM) and observe that the DNA gradually transforms to a complex configuration with an intersection and interwound structures from a circlelike structure. An algorithm is developed to extract the configuration profiles of circular DNA from AFM images and the radius of gyration is used to describe the flexibility of circular DNA. The quantitative analysis of the circular DNA demonstrates that the radius of gyration gradually decreases and two processes on the change of flexibility of circular DNA are found as the cisplatin concentration increases. Furthermore, a model is proposed and discussed to explain the mechanism for understanding the complicated interaction between DNA and cisplatin.
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页数：6
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