Magnetic paper - based ELISA for IgM-dengue detection

被引：34

作者：

Ortega, G. A. ^{[1
,2
]}

Perez-Rodriguez, S. ^{[3
]}

Reguera, E. ^{[1
]}

机构：

[1] IPN, Ctr Appl Sci & Adv Technol, Legaria Unit, Mexico City, DF, Mexico

[2] Univ Havana, Fac Chem, Dept Inorgan Chem, Havana, Cuba

[3] Univ Nacl Autonoma Mexico, Biomed Res Inst, Mexico City, DF, Mexico

来源：

RSC ADVANCES | 2017年 / 7卷 / 09期

关键词：

LINKED-IMMUNOSORBENT-ASSAY; ELECTROCHEMICAL ELISA; ANALYTICAL DEVICES; SERUM SAMPLES; IMMUNOASSAY; BIOSENSOR; NANOPARTICLES; ANTIBODY; ANTIGEN; CAPTURE;

D O I：

10.1039/c6ra25992h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This contribution reports a novel "Magnetic Paper-Based ELISA" using core-shell magnetite@polydopamine nanoparticles supported on a Whatman paper-like new solid immunoassay platform specifically for IgM-dengue antibodies recognition as the proof-of-concept target for antibodies isotype IgM detection. Affordable procedures to deposit magnetite nanoparticles on cellulose paper sheets (Whatman type-1 and ss903) and to conjugate such nanoparticles with anti human-IgM antibodies using polydopamine as linker are reported. Structural features, magnetic behavior, coating homogeneity, and the nanoparticles/linked antibodies ratio were determined. Additionally, "Magnetic Paper-Based ELISA" for IgM-dengue antibodies detection provides a system with improved analytical response (two orders more sensitive with a 700 times lower limit of detection (LOD) than traditional ELISA or using magnetic beads without depositing), appropriate accuracy for real sample detection, lowcost, easymanufacturing, and effortless and easy handling.

引用

页码：4921 / 4932

页数：12

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