A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin

被引：7

作者：

de Santana, M. A. ^{[1
]}

Santos, A. M. C. ^{[1
]}

Oliveira, M. E. ^{[2
]}

de Oliveira, J. S. ^{[1
]}

Baba, E. H. ^{[2
]}

Santoro, M. M. ^{[1
]}

de Andrade, M. H. G. ^{[2
]}

机构：

[1] Univ Fed Minas Gerais, Dept Bioquim & Imunol, Inst Ciencias Biol, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Ouro Preto, Dept Ciencias Biol, Ouro Preto, MG, Brazil

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2008年 / 43卷 / 04期

关键词：

Macrotyloma axillare; Lectin; GalNac; Purification; A1; antigen;

D O I：

10.1016/j.ijbiomac.2008.07.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The N-acetyl-galactosamine specific lectin from Macrotyloma axillare seeds (LMA) was purified by precipitation and ion exchange chromatography. The LMA 0.2 mol L-1 fraction showed hemagglutinating activity on erythrocytes A1. The results for molecular mass determinations were about 28 kDa. The LMA pH-dependent assays showed best hemagglutinating activity at pH 6.0-8.0; being decreased at acidic/alkaline conditions and by EDTA treatment. LMA is a tetramer at pH 8.2 and a dimer at pH 4.0. Human erythrocytes from ABO system confirmed the A1 specificity for LMA. This new methodology is useful and easy, with low costs, for lectin purification in large amounts. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：352 / 358

页数：7

共 31 条

[1]

[Anonymous], 1996, CLIN DIAGNOSIS MANAG

[2] Lectin-mediated drug targeting: history and applications [J].

Bies, C ;

Lehr, CM ;

Woodley, JF .

ADVANCED DRUG DELIVERY REVIEWS, 2004, 56 (04) :425-435

[3] METAL-ION CONTENT OF DOLICHOS-BIFLORUS LECTIN AND EFFECT OF DIVALENT-CATIONS ON LECTIN ACTIVITY [J].

BORREBAECK, CAK ;

LONNERDAL, B ;

ETZLER, ME .

BIOCHEMISTRY, 1981, 20 (14) :4119-4122

[4] Plant toxic proteins with insecticidal properties.: A review on their potentialities as bioinsecticides [J].

Carlini, CR ;

Grossi-de-Sá, MF .

TOXICON, 2002, 40 (11) :1515-1539

[5] Thermodynamic studies of lectin-carbohydrate interactions by isothermal titration calorimetry [J].

Dam, TK ;

Brewer, CF .

CHEMICAL REVIEWS, 2002, 102 (02) :387-429

[6]

DEANDRADE MHG, 2004, Patent No. 200401517

[7] Lectin-carbohydrate interactions: different folds, common recognition principles [J].

Elgavish, S ;

Shaanan, B .

TRENDS IN BIOCHEMICAL SCIENCES, 1997, 22 (12) :462-467

[8] NH2-TERMINAL SEQUENCES OF SUBUNITS OF DOLICHOS-BIFLORUS LECTIN [J].

ETZLER, ME ;

TALBOT, CF ;

ZIAYA, PR .

FEBS LETTERS, 1977, 82 (01) :39-41

[9] PURIFICATION AND CHARACTERIZATION OF A LECTIN (PLANT HEMAGGLUTININ) WITH BLOOD GROUP-A SPECIFICITY FROM DOLICHOS-BIFLORUS [J].

ETZLER, ME ;

KABAT, EA .

BIOCHEMISTRY, 1970, 9 (04) :869-&

[10] ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].

FAIRBANKS, G ;

STECK, TL ;

WALLACH, DFH .

BIOCHEMISTRY, 1971, 10 (13) :2606-+

← 1 2 3 4 →