Rapid Site-Specific Protein Labeling Based on Diels-Alder Bioorthogonal Reactions

被引：4

作者：

Wang Yue ^{[1
]}

Ye Xinshan ^{[1
]}

机构：

[1] Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

来源：

ACTA CHIMICA SINICA | 2012年 / 70卷 / 21期

关键词：

protein labeling; fluorescence probe; bioorthogonal reactions; tetrazine; genetic encoding; unnatural amino acid; IN-VIVO; CHEMISTRY; LIGATION; MOLECULES;

D O I：

10.6023/A12090629

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In recent years, many achievements have been made in site-specific protein modifications. This review highlights the latest advances on protein labeling, which utilizes the inverse electron-demand Diels-Alder bioorthogonal reactions of highly strained alkenes or alkynes and tetrazines, by genetically encoding unnatural amino acids containing one of these functionalities into the specific sites of proteins, leading to the rapid fluorescence labeling of cellular proteins.

引用

页码：2208 / 2212

页数：5

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