MIRA-seq for DNA methylation analysis of CpG islands

被引：24

作者：

Jung, Marc ^{[1
]}

Kadam, Swati ^{[1
]}

Xiong, Wenying ^{[1
]}

Rauch, Tibor A. ^{[2
]}

Jin, Seung-Gi ^{[1
,3
]}

Pfeifer, Gerd P. ^{[1
,3
]}

机构：

[1] City Hope Natl Med Ctr, Beckman Res Inst, Duarte, CA 91010 USA

[2] Rush Univ, Med Ctr, Dept Orthoped Surg, Chicago, IL 60612 USA

[3] Van Andel Res Inst, Grand Rapids, MI 49503 USA

来源：

EPIGENOMICS | 2015年 / 7卷 / 05期

关键词：

CpG islands; DNA methylation; high-throughput sequencing; methyl-CpG-binding protein; methylated-CpG island recovery assay; LUNG-CANCER; TRANSCRIPTIONAL REPRESSOR; BINDING-PROTEINS; RECOVERY ASSAY; GENOME; IDENTIFICATION; CELLS; WIDE; RESOLUTION; METHYLOME;

D O I：

10.2217/epi.15.33

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To develop a reliable method for whole genome analysis of DNA methylation. Materials & methods: Genome-scale analysis of DNA methylation includes affinity-based approaches such as enrichment using methyl-CpG-binding proteins. One of these methods, the methylated-CpG island recovery assay (MIRA), is based on the high affinity of the MBD2b-MBD3L1 complex for CpG-methylated DNA. Here we provide a detailed description of MIRA and combine it with next generation sequencing platforms (MIRA-seq). Results: We assessed the performance of MIRA-seq and compared the data with whole genome bisulfite sequencing. Conclusion: MIRA-seq is a reliable, genome-scale DNA methylation analysis platform for scoring DNA methylation differences at CpG-rich genomic regions. The method is not limited by primer or probe design and is cost effective.

引用

页码：695 / 706

页数：12

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