Comparative Studies on Isolation of Medium-chain-length Polyhydroxyalkanoates Produced by Pseudomonas spp. Strains

被引：0

作者：

Lupescu, Irina ^{[1
,2
]}

Eremia, Mihaela Carmen ^{[1
]}

Savoiu, Gabriela Valeria ^{[1
]}

Spiridon, Maria ^{[1
]}

Panaitescu, Denis ^{[3
]}

Nicolae, Cristian ^{[3
]}

Vladu, Mariana Gratiela ^{[1
]}

Stefaniu, Amalia ^{[1
]}

机构：

[1] Natl Inst Chem Pharmaceut Res & Dev ICCF Buchares, 112 Vitan Ave, Bucharest 031299, Romania

[2] Spiru Haret Univ, Fac Vet Med, 9-11 Energeticienilor Blvd, Bucharest 32091, Romania

[3] Natl Inst Res & Dev Chem & Petrochem, 202 Spl Independentei, Bucharest 060021, Romania

来源：

REVISTA DE CHIMIE | 2016年 / 67卷 / 10期

关键词：

Medium chain length polyhydroxyalkanoates; Pseudomonas spp; extraction; BIOSYNTHESIS; RECOVERY; PHA;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Several methods were used for recovering medium-chain-length polyhydroxyalkanoates (mcl-PHAs) biosynthesized by a Pseudomonas spp. strain cells, in a fermentation medium based on sodium citrate and sodium octanoate. To improve bacterial cells separation, flocculation was tested, using a anionic polyelectrolyte. Post-biosynthesis processing of separated biomass included cell rupture and solubilization of all cell materials except PHAs and/or solvent extraction of PHAs. NaOCl or lysozyme + EDTA were used for biomass digestion, CHCl3 and acetone for polyester extraction. The isolated polymers were characterized by GC-FID, FTIR, and DSC-TGA. The best results were obtained using acetone Soxhlet extraction from dried cells. Thus, mcl-PHAs were recovered as thin polymeric membranes, of above 99% purity, representing 44-54% of dry bacterial cells, and containing C6, C8 and C10 hydroyacid monomers in a gravimetric percentage of: 9.50 - 11.47% C6, 85.25 - 86.75% C8 and 1.85 - 3.01% C10.

引用

页码：1957 / 1962

页数：6

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