A new fluorescent probe, ABM: Properties and application in clinical diagnostics

被引：14

作者：

Kalnina, I ^{[1
]}

Meirovics, I ^{[1
]}

机构：

[1] Riga Tech Univ, Fac Chem Technol, LV-1048 Riga, Latvia

来源：

JOURNAL OF FLUORESCENCE | 1999年 / 9卷 / 01期

关键词：

fluorescent probe; lymphocytes; diagnostics;

D O I：

10.1023/A:1020579522498

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Properties of benzanthrone aminoderivative ABM (conditional name) as a potential fluorescent probe were investigated. Spectral characteristics of the compound in different solvents as well as their binding to model Lipid membranes (Liposomes) and human peripheral blood mononuclear cells (lymphocytes; ly) were determined. The fluorescence was found to be sensitive polarity changes to the environment. Distinctions were observed in the spectral characteristics of the investigated compound when bounded to Liposomes. It was established that spectral characteristics of ABM in cell suspension qualitatively characterize the structural and functional alterations of ly during pathological phenomena and correlate directly with the clinical view of disease. The ABM is shown to be a perspective in the screening for various pathologies.

引用

页码：27 / 32

页数：6

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