Studies on primer-dimer formation in polymerase chain reaction (PCR)

被引：27

作者：

Das, S ^{[1
]}

Mohapatra, SC ^{[1
]}

Hsu, JT ^{[1
]}

机构：

[1] Lehigh Univ, Dept Chem Engn, Bethlehem, PA 18015 USA

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 10期

关键词：

mechanistic; PCR; primer-dimer; Taq DNA polymerase enzyme;

D O I：

10.1023/A:1008924615839

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A viable mechanism for primer-dimer formation in polymerase chain reaction (PCR) process has been proposed based on experimental results. This mechanism results in a kinetic description of the primer-dimer formation process with the Taq DNA polymerase enzyme, the two primers and the dNTPs as the starting materials. The possible impact of this primer-dimer formation on the selectivity and yield of PCR process is discussed.

引用

页码：643 / 646

页数：4

共 6 条

[1]

BROWNIE J, 1998, J BIOCH, V10, P233

[2]

Hsu JT, 1997, BIOTECHNOL BIOENG, V55, P359, DOI 10.1002/(SICI)1097-0290(19970720)55:2<359::AID-BIT13>3.0.CO

[3]

2-C

[4] OPTIMIZATION OF THE ANNEALING TEMPERATURE FOR DNA AMPLIFICATION INVITRO [J].