Profiling of environmental Legionella pneumophila strains by randomly amplified polymorphic DNA method isolated from geographically nearby buildings

被引:6
作者
Zeybek, Z. [1 ]
Turetgen, Irfan [1 ]
Erdem, Ayten Kimiran [1 ]
Filoglu, Goenuel [2 ]
Cotuk, Aysin [1 ]
机构
[1] Istanbul Univ, Dept Biol, Fac Sci, Istanbul, Turkey
[2] Istanbul Univ, Inst Forens Sci, Istanbul, Turkey
关键词
Legionella pneumophila; RAPD analysis; IDENTIFICATION;
D O I
10.1007/s10661-008-0205-x
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Legionella pneumophila (L. pneumophila) which is also known as etiologic agent Legionnaires Disease lives in natural water and man made water systems. These bacteria belonging to Legionellaceae family are divided 15 serogroups. Phenotypical methods used for the identification of Legionella isolates are not very discriminatory. In this study we investigated genotypic features of eight L. pneumophila serogroup 1 and 18 L. pneumophila serogroup 2-14 strains isolated from different buildings in Istanbul by randomly amplified polymorphic DNA (RAPD) method. Eight L. pneumophila serogroup 1 strains (37.5%) were similar RAPD profile and they were isolated from buildings located in a short distance (about 500 m). Four L. pneumophila serogroup 2-14 strains (22%) were identical genotypically. Three of these strains were isolated from buildings located in a short distance.
引用
收藏
页码:323 / 327
页数:5
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